#single-molecule, #super-res, #ion-imaging, #confocal, #quantum, #astronomy, #luminescence
Andor

iXon EMCCD Cameras

With the iXon EMCCD cameras, Andor have delivered a dedicated, truly high-end, yet accessible ultrasensitive scientific camera platform, designed specifically to drive the absolute best from EMCCD technology across all critical performance specs and parameters.

NEW
  iXon Life – Exclusively for Fluorescence Microscopy
  • Single photon sensitivity
  • Back-illuminated > 95% QE
  • Fast frame rates
  • Unique price/performance
  • The ultimate detector for single molecules
  • Preserve accurate cell physiology

EMCCD Applications

Single Molecule
Ion Imaging
Confocal
Quantum
Astronomy
Luminescence



Single Molecule Detection

For many years, Andor’s iXon EMCCDs have been the gold standard detectors of the biophysics laboratory. To this day they remaining the dominant detector type, operating in a low light regime that is less suited to even back-illuminated sCMOS cameras, especially under the critically demanding conditions of minimized fluorophore photobleaching.

The accelerated readout rates of the iXon Life, especially combined with ‘Optically Centred Crop Mode’, means that dynamic single molecule processes can be better characterised.

The 13μm pixel of the 888 model provides superb single molecule resolving capability at the diffraction limit, while preserving optical photon collection efficiency.

Distinctions between iXon Ultra and iXon Life models

iXon Ultra

Driving the absolute best from EMCCD technology

The market leading EMCCD camera, Andor’s iXon Ultra has been long recognised as the highest performance, most versatile solution for the most demanding of light starved applications. Available for both physical and life science applications, iXon Ultra delivers unbeatable flexibility and functionality, offering market leading TE cooling, ‘2-in-1’ EMCCD and CCD readout modes and a host off other high end functionality.

NEW

iXon Life

EMCCD Performance, sCMOS Price!

Andor’s new iXon Life EMCCD platform is available exclusively for fluorescence microscopy applications and is engineered to deliver single photon sensitivity with absolutely unparalleled price/performance. Perfect for single molecule detection and live cell microscopy with minimized phototoxicity or photobleaching, but at a price more normally associated with back-illuminated sCMOS cameras.

  iXon Ultra iXon Life
Conventional CCD Amplifier -
Integrated Shutter -
Minimum TE Cooling Temperature -100 °C -80 °C
Direct Data Access (CameraLink) -
EMCCD Mode Readout speeds (MHz) 888 model - 30, 20, 10 & 1
897 model - 17, 10, 5 & 1
888 model - 30 & 10
897 model - 17 & 10
Single Photon Counting Modes -
Dual-AR QE (EX2) and Fringe Suppression options -
Window coating choices -

iXon model options

Each of the model types listed are available in both 'Ultra' and 'Life' platforms

Models iXon 888 iXon 897
Core attributes Field of view, sensitivity and speed Sensitivity and speed
Sensor format 1024 x 1024 512 x 512
Sensor diagonal 18.8 mm 11.6 mm
QE options BV (Life) or BV, EX2, UVB (Ultra) BV (Life) or BV, EX2, UVB (Ultra)
Pixel Size 13 µm 16 µm
Frame Rate 26 fps (670 fps with 128 x 128 Crop Mode) 56 fps (595 fps with 128 x 128 Crop Mode)
Read Noise < 1 e- with EM Gain < 1 e- with EM Gain
Pixel well depth 80,000 e- 180,000 e-
Interface USB 3.0 USB 2.0
Learn more ULTRA SPEC SHEET LIFE SPEC SHEET ULTRA SPEC SHEET LIFE SPEC SHEET
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Benefit from the Best

EMCCD: the best technology
iXon: the best camera
Solutions: the best for your application



EMCCD: the best technology

The Electron Multiplying CCD (EMCCD) operates by amplification (EM Gain) of weak signal events to a signal level that is well clear of the read noise floor of the camera at any readout speed, rendering them single photon sensitive.

While recent innovations in high QE, low noise sCMOS technology are certainly welcomed for broad scientific usage, only EMCCDs can deliver the raw sensitivity required for the most light starved conditions, and the key application benefits that this unlocks.

Customer Publications

Author Title Year
Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
Real-time Quantification of Single RNA Translation Dynamics in Living Cells
Photometry unlocks 3D information from 2D localization microscopy data
Dynamic Filament Formation by a Divergent Bacterial Actin-Like ParM Protein
Imaging potassium-flux through individual electropores in droplet interface bilayers
Expansion microscopy with conventional antibodies and fluorescent proteins
Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy
Low-cost multimodal light sheet microscopy for optically cleared tissues and living specimens
Distinct stages in stress granule assembly and disassembly
Massive and parallel expression profiling using microarrayed single-cell sequencing
SuReSim: simulating localization microscopy experiments from ground truth models
Correlative super-resolution imaging of RNA polymerase distribution and dynamics, bacterial membrane and chromosomal structure in Escherichia coli
Cell-specific STORM super-resolution imaging reveals nanoscale organization of cannabinoid signaling
Spatially resolved, highly multiplexed RNA profiling in single cells
Natural Loss of Mps1 Kinase in Nematodes Uncovers a Role for Polo-like Kinase 1 in Spindle Checkpoint Initiation
GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane
The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function