Cell Motility - An overview of Andor's solutions for Cell Motility

Cell Motility

An overview of Andor's solutions for Cell Motility

Cell motility is required for many important physiological processes during development, such as cell migration during gastrulation, axon guidance, tissue regeneration and embryological development. Unregulated cell migration can be the cause for progression of cancer, e.g during metastasis. The challenge to perform rapid, multi-dimensional imaging of motile cells is fundamental to our understanding of above mentioned processes.At the level of single cell visualization, cell motility envelopes a broad area of study including the mechanisms of cell migration, chemotaxis, axon guidance and motility of dendritic spines. Of interest are whole cell movement, cell polarity, adhesion, membrane ruffles, protrusion of lamellipodia and filopodia, morphogenesis and also the involvement of the cytoskeleton, particularly at the leading and trailing edges of locomotion. Historically the microscopy of motile cells has, from the instrument standpoint, been marred by the need for greater speed and sensitivity at high resolution. For example, it can be desirable to image rapid protrusion of lamellipodia and filopodia.It can also be fundamental to visualize the cytoskeletal dynamics and membrane morphology of moving cells with high resolution and sensitivity, such that the underlying mechanisms of protrusion and retraction can be understood in the context of the interactions and growth of actin (e.g. stress fibers), mictrotubule and intermediate filament cytoskeletons.Underlying all direct imaging studies of living cells or organisms, is the desire to preserve the living subject for as long as possible, through minimization of both phototoxic cell/tissue damage and photobleaching of the incorporated fluorophores.

The iXon3 EMCCD Camera has provided the solution to the challenges described above, enabling high resolution, high signal-to-noise (S/N) movies to be acquired of cell systems and their chemotactic response, without sacrificing any of the critical imaging parameters. Furthermore, through reducing the excitation power, phototoxic effects are minimized, enabling cells to be followed for much longer periods. These cameras are also the perfect partner to live cell confocal solutions such as Revolution XD and Revolution WD.

iXon and Luca imaging EMCCD platforms each display single photon sensitivity combined with high Quantum Efficiency (QE) at multi-MHz rapid readout speeds.

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Publications Database
The yeast Ste2p G protein-coupled receptor dimerizes on the cell plasma membrane
Amphiphilic Ferrocene-Containing PEG Block Copolymers as Micellar Nanocarriers and Smart Surfactants
Poly(glycerol sebacate) nanoparticles for encapsulation of hydrophobic anti-cancer drugs
Contrast Induced by a Static Magnetic Field for Improved Detection in Nanodiamond Fluorescence Microscopy
Heterogeneous expression of extracellular matrix molecules in the red nucleus of the rat
Flow of wormlike micellar solutions around confined microfluidic cylinders
Lateral Microscope Enables the Direct Observation of Cellular Interfaces and Quantification of Changes in Cell Morphology during Adhesion
Expansion and concatenation of nonmuscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis
Lung Microtissue Array to Screen the Fibrogenic Potential of Carbon Nanotubes
β-glucan microparticles targeted to epithelial APN as oral antigen delivery system
A Generic Polymer–Protein Ligation Strategy for Vaccine Delivery
Dyes as bifunctional markers of DNA hybridization on surfaces and mutation detection
Submicron thermal imaging of a nucleate boiling process using fluorescence microscopy
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Transient submicron temperature imaging based on the fluorescence emission in an Er/Yb co-doped glass–ceramic
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
Anisotropic stress orients remodelling of mammalian limb bud ectoderm
An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms
Gap geometry dictates epithelial closure efficiency
The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function

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