Cell Motility

An overview of Andor's solutions for Cell Motility

Cell motility is required for many important physiological processes during development, such as cell migration during gastrulation, axon guidance, tissue regeneration and embryological development. Unregulated cell migration can be the cause for progression of cancer, e.g during metastasis. The challenge to perform rapid, multi-dimensional imaging of motile cells is fundamental to our understanding of above mentioned processes.At the level of single cell visualization, cell motility envelopes a broad area of study including the mechanisms of cell migration, chemotaxis, axon guidance and motility of dendritic spines. Of interest are whole cell movement, cell polarity, adhesion, membrane ruffles, protrusion of lamellipodia and filopodia, morphogenesis and also the involvement of the cytoskeleton, particularly at the leading and trailing edges of locomotion. Historically the microscopy of motile cells has, from the instrument standpoint, been marred by the need for greater speed and sensitivity at high resolution. For example, it can be desirable to image rapid protrusion of lamellipodia and filopodia.It can also be fundamental to visualize the cytoskeletal dynamics and membrane morphology of moving cells with high resolution and sensitivity, such that the underlying mechanisms of protrusion and retraction can be understood in the context of the interactions and growth of actin (e.g. stress fibers), mictrotubule and intermediate filament cytoskeletons.Underlying all direct imaging studies of living cells or organisms, is the desire to preserve the living subject for as long as possible, through minimization of both phototoxic cell/tissue damage and photobleaching of the incorporated fluorophores.

The iXon3 EMCCD Camera has provided the solution to the challenges described above, enabling high resolution, high signal-to-noise (S/N) movies to be acquired of cell systems and their chemotactic response, without sacrificing any of the critical imaging parameters. Furthermore, through reducing the excitation power, phototoxic effects are minimized, enabling cells to be followed for much longer periods. These cameras are also the perfect partner to live cell confocal solutions such as Revolution XD and Revolution WD.

iXon and Luca imaging EMCCD platforms each display single photon sensitivity combined with high Quantum Efficiency (QE) at multi-MHz rapid readout speeds.

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Publications Database
Submicron thermal imaging of a nucleate boiling process using fluorescence microscopy
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Transient submicron temperature imaging based on the fluorescence emission in an Er/Yb co-doped glass–ceramic
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
Anisotropic stress orients remodelling of mammalian limb bud ectoderm
An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms
Gap geometry dictates epithelial closure efficiency
The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function
Temporal sequence of activation of cells involved in purinergic neurotransmission in the colon
In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy
Direct interaction between centralspindlin and PRC1 reinforces mechanical resilience of the central spindle
The role of Ca2+ influx in spontaneous Ca2+ wave propagation in interstitial cells of Cajal from the rabbit urethra
A mouse model of human primitive neuroectodermal tumors resulting from microenvironmentally-driven malignant transformation of orthotopically transplanted radial glial cells.
Neuronal activity and AMPA-type glutamate receptor activation regulates the morphological development of oligodendrocyte precursor cells
Systematic imaging reveals features and changing localization of mRNAs in Drosophila development
Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity
Functionalized fluorescent silver nanoparticle surfaces for novel sensing and imaging techniques
Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis

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