Filter Exchange & Calibration for the Revolution DSD
A technical overview...
Andor’s unique design of the Revolution system allows the DSD to remain fixed to the microscope and the camera fixed to the DSD during filter turret switching. This removes any requirement to re-align the optical and mechanical assembly. The filter turrets, shown right, contain the excitation, dichroic, and emission filters for the DSD.
The images below show the actions required to change the filter turret. Remove the existing filter turret by lifting the lid and removing 3 screws (1-5). Insert new set (6) and repeat steps 1 to 5 in reverse. To guide you through the process, a Filter Change Wizard in Andor iQ, provides step by step instructions.
In addition to the turret, each DSD filter set has a multi-band emission filter, which is inserted into the tube connecting the DSD to the Clara CCD camera.
The ability to exchange multiband emission filters without removing the camera from the system allows for more rapid system configuration and is unique to the Revolution DSD.
The last step in changing the DSD filter set is to run a calibration in Andor iQ. This can be done with a single mouse click and no user intervention is required to complete the process, which takes just one minute.
||Excitation & Emission Filter Pairs Centre/FWHM
||457/50 & 525/50, 556/20 & 617/73
||427/10 & 475/50, 500/24 & 542/50
||CFP, YFP, CFPYFP
||534/36 & 577/24, 635/31 & 690/50
||Cy3, Alexa 555, Cy5, Alexa 647
||406/15 & 457/50, 494/20 & 536/40, 575/25 & 628/40
||406/15, 494/20, 575/25
||DAPI, FITC, Texas Red
||387/11 & 440/40, 485/20 & 521/21, 560/25 & 607/34, 650/13 & 700/45
||387/11, 485/20, 560/25, 650/13
||DAPI, FITC, TRITC, Cy5
||On Special Request
Revolution DSD supports light sources with fast wavelength and intensity switching, giving three distinct advantages.
• DSD offers four or more wavelengths in contrast to a standard filter turret
• DSD wavelength switching times are much reduced
• Instrumental background is reduced because only desired source wavelengths reach the DSD