Imaging Protein Dynamics in Living Cells - A Novel Approach

Timothy J. Stasevich - July 2015

Title: Imaging Protein Dynamics in Living Cells - A Novel Approach

Speakers: Prof. Tim Stasevich (Biochemistry & Molecular Biology, Colorado State University) & Dr Orla Hanrahan (Life Science Application Specialist, Andor)


Professor Stasevich presented findings from his research and how using a combination of multiplexed single-molecule tracking, photoactivatable dyes and quantitative analysis. He also discussed how he is using this technology to map histone and other protein modifications across the genome with unprecedented spatio-temporal resolution in single living cells.

Dr Orla Hanrahan (Life Science Application Specialist, Andor Technology) discussed the world’s first Megapixel back-illuminated EMCCD camera - the iXon Ultra 888. This camera offers exceptional frame rates and single photon sensitivity across a large field of view. The ‘supercharged’ iXon Ultra 888, represents a massive performance boost for the largest available EMCCD sensor, as well as the first USB3 enabled EMCCD camera.

Key Learning Objectives

• Imaging protein dynamics in living cells – benefits and challenges
• Why EMCCD technology is required for single molecule imaging
• Features and benefits of the new EMCCD will be discussed in detail 

Multimedia Library
Application Images (4)
Application Movies (3)
Publications Database
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Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
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Single Molecule Studies of Telomere DNA
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Heterogeneous Intracellular Trafficking Dynamics of Brain-Derived Neurotrophic Factor Complexes in the Neuronal Soma Revealed by Single Quantum Dot …
Imaging Approach to Mechanistic Study of Nanoparticle Interactions with the BloodÀBrain Barrier
Optimising the signal-to-noise ratio in measurement of photon pairs with detector arrays
Statistics of twin-beam states by photon-number resolving detectors up to pump depletion
The Einstein-Podolsky-Rosen paradox in twin images
Spatial properties of twin-beam correlations at low-to high-intensity transition
Coherence properties of high-gain twin beams generated in pump-depletion regime
H-Ras forms dimers on membrane surfaces via a protein–protein interface
Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization toward higher-order …
A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment

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