Imaging Protein Dynamics in Living Cells | Andor

Imaging Protein Dynamics in Living Cells - A Novel Approach

Timothy J. Stasevich - July 2015

Title: Imaging Protein Dynamics in Living Cells - A Novel Approach

Speakers: Prof. Tim Stasevich (Biochemistry & Molecular Biology, Colorado State University) & Dr Orla Hanrahan (Life Science Application Specialist, Andor)


Professor Stasevich presented findings from his research and how using a combination of multiplexed single-molecule tracking, photoactivatable dyes and quantitative analysis. He also discussed how he is using this technology to map histone and other protein modifications across the genome with unprecedented spatio-temporal resolution in single living cells.

Dr Orla Hanrahan (Life Science Application Specialist, Andor Technology) discussed the world’s first Megapixel back-illuminated EMCCD camera - the iXon Ultra 888. This camera offers exceptional frame rates and single photon sensitivity across a large field of view. The ‘supercharged’ iXon Ultra 888, represents a massive performance boost for the largest available EMCCD sensor, as well as the first USB3 enabled EMCCD camera.

Key Learning Objectives

• Imaging protein dynamics in living cells – benefits and challenges
• Why EMCCD technology is required for single molecule imaging
• Features and benefits of the new EMCCD will be discussed in detail 

Multimedia Library
Application Images (5)
Application Movies (4)
Publications Database
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
Neuronal serotonin release triggers the heat shock response in C. elegans in the absence of temperature increase
In situ biosynthesis of fluorescent platinum nanoclusters: Towards self-bio-imaging guided cancer theranostics
Single Molecule Studies of Telomere DNA
Super-resolution imaging of C-type lectin spatial rearrangement within the dendritic cell plasma membrane at fungal microbe contact sites
Point spread function optimization for STORM using adaptive optics
Method for co-cluster analysis in multichannel single-molecule localisation data
Heterogeneous Intracellular Trafficking Dynamics of Brain-Derived Neurotrophic Factor Complexes in the Neuronal Soma Revealed by Single Quantum Dot …
Imaging Approach to Mechanistic Study of Nanoparticle Interactions with the BloodÀBrain Barrier
Optimising the signal-to-noise ratio in measurement of photon pairs with detector arrays
Statistics of twin-beam states by photon-number resolving detectors up to pump depletion
The Einstein-Podolsky-Rosen paradox in twin images
Spatial properties of twin-beam correlations at low-to high-intensity transition
Coherence properties of high-gain twin beams generated in pump-depletion regime
H-Ras forms dimers on membrane surfaces via a protein–protein interface
Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization toward higher-order …
A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment

Sign up for the Andor Newsletter Now!

Receive articles like this one, product launches, press releases and more with our quarterly newsletter focusing on either Physical Science or Life Science. It's free to subscribe and you can opt out at any time.


Physical ScienceLife ScienceSUBMIT