Imaging Protein Dynamics in Living Cells - A Novel Approach
Timothy J. Stasevich - July 2015
Title: Imaging Protein Dynamics in Living Cells - A Novel Approach
Speakers: Prof. Tim Stasevich (Biochemistry & Molecular Biology, Colorado State University) & Dr Orla Hanrahan (Life Science Application Specialist, Andor)
Professor Stasevich presented findings from his research and how using a combination of multiplexed single-molecule tracking, photoactivatable dyes and quantitative analysis. He also discussed how he is using this technology to map histone and other protein modifications across the genome with unprecedented spatio-temporal resolution in single living cells.
Dr Orla Hanrahan (Life Science Application Specialist, Andor Technology) discussed the world’s first Megapixel back-illuminated EMCCD camera - the iXon Ultra 888. This camera offers exceptional frame rates and single photon sensitivity across a large field of view. The ‘supercharged’ iXon Ultra 888, represents a massive performance boost for the largest available EMCCD sensor, as well as the first USB3 enabled EMCCD camera.
Key Learning Objectives
• Imaging protein dynamics in living cells – benefits and challenges
• Why EMCCD technology is required for single molecule imaging
• Features and benefits of the new EMCCD will be discussed in detail