Light Sheet Microscopy

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Over the past 10 years, a new fluorescence microscopy technique has come to the fore that is faster and less phototoxic than other microscopy techniques, making it ideal for studying living organisms and the biological processes that take place within them. Known as light-sheet microscopy (LSM) or selective plane illumination microscopy (SPIM), its key feature is that the illumination is done perpendicularly to the direction of observation. The expanded beam of a laser is focused in only one direction by a cylindrical lens, or by a combination of a cylindrical lens and a microscope objective, as the latter is available in better optical quality and with higher numerical aperture (NA) than the first. In this way a thin sheet of light is created in the focal region that can be used to excite fluorescence only in a thin slice (usually a few micrometers) of the sample.

Application Notes and Case Studies
SPIM

Model systems in developmental biology use a variety of transgenic animals in which fluorescent proteins label individual cells, particular tissues or whole embryos. Such fluorescent transgenic organisms offer the opportunity to visualize cell and tissue behaviour during development...

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Using Inverted SPIM (iSPIM) to study neural development in C. elegans

Inverted Selective Plane Illumination Microscopy (iSPIM) is a type of light sheet fluorescence microscopy technique developed by Yicong Wu and Hari Shroff (both at the National Institute of Biomedical Imaging and Bioengineering (NIBIB)) and their colleagues at Yale University and...

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Light Sheet Microscopy

The entire development of the nervous system can now be viewed with excellent temporal resolution using the exceptional capture rate of the Neo sCMOS combined with the Light Sheet Microscopy technique.

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Fundamentals of Embryo Development uncovered in real time

Jan Huisken and his research team (MPI, Dresden) took advantage of the light sheet microscopy approach of SPIM and the fast acquisition speed of the sCMOS camera to study the endoderm of the Zebrafish during early stages of development.

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SPIM Multimedia

Multimedia Library
Application Images (4)
Application Movies (11)
Publications Database
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
Bessel beam plane illumination microscope
Optimising the signal-to-noise ratio in measurement of photon pairs with detector arrays
Statistics of twin-beam states by photon-number resolving detectors up to pump depletion
The Einstein-Podolsky-Rosen paradox in twin images
Spatial properties of twin-beam correlations at low-to high-intensity transition
Coherence properties of high-gain twin beams generated in pump-depletion regime
High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics
Towards comprehensive cell lineage reconstructions in complex organisms using light‐sheet microscopy
Compartmentalized Calcium Transients Trigger Dendrite Pruning in Drosophila Sensory Neurons
The influence of non-imaging detector design on heralded ghost-imaging and ghost-diffraction examined using a triggered ICCD camera
Light Sheet Microscopy for Tracking Single Molecules on the Apical Surface of Living Cells
Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope
Multiview light-sheet microscope for rapid in toto imaging
Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy
Multiview light-sheet microscope for rapid in toto imaging
Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy
High‐resolution live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy
Deep and fast live imaging with two-photon scanned light-sheet microscopy

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