Live Cell Confocal Microscopy

An overview of Andor's solutions for Live Cell

Live Cell Confocal Microscopy
A neuron image stack recorded with Revolution 488, rendered topographically in Andor iQ. The image represents a height coded image of the specimen, with red being closest to the viewer and blue being furthest away. Data courtesy of Dr Tom Blanpied, University of Maryland –formerly of Duke University.

Andor Revolution provides a framework for Andor Bioimaging Division's laser spinning disk, live cell confocal microscopy solutions, which combine our own award winning iXon3 EMCCD Camera with the renowned Yokogawa CSUX units. Andor has a global distribution agreement with Yokogawa Electric Corporation, to integrate powerful confocal solutions. This partnership brings you unrivalled performance, product understanding and support.Revolution encompasses a range of complimentary components, both hardware and software, that fit seamlessly together creating a complete confocal microscopy solution. A flexible component focus also allows us to provide key pieces of hardware stand-alone.At the core of Revolution systems is Andor iQ, a multi-dimensional imaging software which synchronizes iXon cameras with the CSUX spinning disk confocal and other key hardware components such as Piezo Z100 fast z-stage and our Solid State Laser Combiner, with Acousto-Optical Tunable Filter (AOTF) for rapid laser line selection.Our recommended microscope platform is the Olympus IX81, optimized for live cell imaging, but infinity-corrected microscopes from Leica, Nikon, Till or Zeiss can also be supported.

Andor provides camera-based solutions for low-light live cell microscopy that are primarily based around ultrasensitive and fast EMCCD technology.Andor's cutting-edge Revolution Multi-dimensional Confocal Microscopy solutions are ideal for demanding dynamic live-cell techniques, including: Cell Motility Cytoskeleton Dynamics Embryo Development Fluorescent Resonance Energy Transfer (FRET) GFP expression Intracellular Ion Signalling Vesicle Transport

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Application Movies (61)
Publications Database
Submicron thermal imaging of a nucleate boiling process using fluorescence microscopy
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Transient submicron temperature imaging based on the fluorescence emission in an Er/Yb co-doped glass–ceramic
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
Anisotropic stress orients remodelling of mammalian limb bud ectoderm
An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms
Gap geometry dictates epithelial closure efficiency
The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function
Temporal sequence of activation of cells involved in purinergic neurotransmission in the colon
In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy
Direct interaction between centralspindlin and PRC1 reinforces mechanical resilience of the central spindle
The role of Ca2+ influx in spontaneous Ca2+ wave propagation in interstitial cells of Cajal from the rabbit urethra
A mouse model of human primitive neuroectodermal tumors resulting from microenvironmentally-driven malignant transformation of orthotopically transplanted radial glial cells.
Neuronal activity and AMPA-type glutamate receptor activation regulates the morphological development of oligodendrocyte precursor cells
Systematic imaging reveals features and changing localization of mRNAs in Drosophila development
Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity
Functionalized fluorescent silver nanoparticle surfaces for novel sensing and imaging techniques
Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis
Signal inhibition by a dynamically-regulated pool of mono-phosphorylated MAPK
Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function

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