Single Molecule Imaging - Applications To Cellular Processes

Single Molecule Imaging - Applications To Cellular Processes

March 2017

Using high-affinity antibody-based probes, multimerized epitope tags, and single molecule microscopy, we are able to image single RNA translation dynamics in living cells and quantify the emergence of nascent protein.

This technology will be described as well as a two color extension, useful for comparing translation rates between two different parts of a single open reading frame (ORF) or two different ORFs. Using information from the correlations of fluorescence fluctuations, we can accurately quantify single mRNA translation elongation rates in both tagged and untagged portions of ORFs. By transiently loading probes and reporter DNA into cells in a combinatorial fashion, multiplexed imaging of gene expression is possible.

This webinar will introduce the new iXon Life EMCCD cameras and provide performance comparison with the well-known iXon Ultra cameras. Both camera series are the best-in-class detectors for sensitivity allowing short exposure times and thus reduced photo-toxicity and photo-bleaching. Used in ‘crop mode’, EMCCDs can image at high frame rate with e.g. 93 fps for a sub-array of 512 x 512 pixels.

Multimedia Library
Application Images (3)
Application Movies (2)
Publications Database
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
Single Molecule Studies of Telomere DNA
Super-resolution imaging of C-type lectin spatial rearrangement within the dendritic cell plasma membrane at fungal microbe contact sites
Point spread function optimization for STORM using adaptive optics
Method for co-cluster analysis in multichannel single-molecule localisation data
Heterogeneous Intracellular Trafficking Dynamics of Brain-Derived Neurotrophic Factor Complexes in the Neuronal Soma Revealed by Single Quantum Dot …
Imaging Approach to Mechanistic Study of Nanoparticle Interactions with the BloodÀBrain Barrier
Optimising the signal-to-noise ratio in measurement of photon pairs with detector arrays
Statistics of twin-beam states by photon-number resolving detectors up to pump depletion
The Einstein-Podolsky-Rosen paradox in twin images
Spatial properties of twin-beam correlations at low-to high-intensity transition
Coherence properties of high-gain twin beams generated in pump-depletion regime
H-Ras forms dimers on membrane surfaces via a protein–protein interface
Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization toward higher-order …
A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment
Data Analysis for Single-Molecule Localization Microscopy
Correlative live-cell and superresolution microscopy reveals cargo transport dynamics at microtubule intersections

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