Single Molecule Imaging with Multiple Wavelengths

Elsevier Webinar - May 2015

This webinar showcased two different imaging techniques used to visualize multiple fluorescent single molecules simultaneously. The vast majority of fluorescence microscopy applications involve the use of more than one fluorescent probe e.g. FRET, dual colour imaging, co-localization studies, etc. The ability to detect multiple fluorescent targets simultaneously can enable visualization of complex functional and molecular processes in-vivo.

Key learning objectives:

  • Single molecule methods leading to the group of super-resolution techniques based on fluorophore localisation
  • Multicolour single molecule imaging over a large field of view
  • Extending single molecule imaging to live animals
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Publications Database
Submicron thermal imaging of a nucleate boiling process using fluorescence microscopy
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Transient submicron temperature imaging based on the fluorescence emission in an Er/Yb co-doped glass–ceramic
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
Anisotropic stress orients remodelling of mammalian limb bud ectoderm
An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms
Gap geometry dictates epithelial closure efficiency
The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function
Temporal sequence of activation of cells involved in purinergic neurotransmission in the colon
In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy
Direct interaction between centralspindlin and PRC1 reinforces mechanical resilience of the central spindle
The role of Ca2+ influx in spontaneous Ca2+ wave propagation in interstitial cells of Cajal from the rabbit urethra
A mouse model of human primitive neuroectodermal tumors resulting from microenvironmentally-driven malignant transformation of orthotopically transplanted radial glial cells.
Neuronal activity and AMPA-type glutamate receptor activation regulates the morphological development of oligodendrocyte precursor cells
Systematic imaging reveals features and changing localization of mRNAs in Drosophila development
Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity
Functionalized fluorescent silver nanoparticle surfaces for novel sensing and imaging techniques
Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis
Signal inhibition by a dynamically-regulated pool of mono-phosphorylated MAPK
Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function

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