Studying laser microirradiation induced DNA damage and repair using live cell microscopy

March 2013

Dr. Gunjan provides a detailed overview of the hardware and the methodologies involved in the study of laser microirradiation induced DNA damage and aims to enable other investigators to take advantage of these improved techniques for the study of DNA damage and repair.

DOWNLOAD SLIDES

Relevant Material

Multimedia Library
Application Images (17)
Show more
Application Movies (34)
Publications Database
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms
Gap geometry dictates epithelial closure efficiency
The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function
Direct interaction between centralspindlin and PRC1 reinforces mechanical resilience of the central spindle
Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity
Functionalized fluorescent silver nanoparticle surfaces for novel sensing and imaging techniques
Signal inhibition by a dynamically-regulated pool of mono-phosphorylated MAPK
Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function
A High Throughput Micro-Chamber Array Device for Single Cell Clonal Cultivation and Tumor Heterogeneity Analysis
Natural Loss of Mps1 Kinase in Nematodes Uncovers a Role for Polo-like Kinase 1 in Spindle Checkpoint Initiation
A geminivirus betasatellite damages the structural and functional integrity of chloroplasts leading to symptom formation and inhibition of photosynthesis
Potent and selective small-molecule MCL-1 inhibitors demonstrate on-target cancer cell killing activity as single agents and in combination with ABT-263 (navitoclax)
RGS Proteins and Septins Cooperate to Promote Chemotropism by Regulating Polar Cap Mobility
A mechanical microcompressor for high resolution imaging of motile specimens
Motif‐Designed Peptide Nanofibers Decorated with Graphene Quantum Dots for Simultaneous Targeting and Imaging of Tumor Cells
In situ biosynthesis of fluorescent platinum nanoclusters: Towards self-bio-imaging guided cancer theranostics
Automated Analysis of Cell-Matrix Adhesions in 2D and 3D Environments
Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters
Speaker

Akash Gunjan, Ph.D.

Assistant Professor, Department of Biomedical Sciences, Florida State University College of Medicine

Dr. Gunjan obtained his Bachelor’s degree with a triple major in Genetics, Chemistry and Zoology from Nizam College in India. He then moved to the US to obtain his Ph.D. degree in Biochemistry at the University of Mississippi Medical Center where he contributed to pioneering studies on the dynamics of chromatin proteins in living cells (Nature, 408: 877-881). He then moved to England to carry out his post-doctoral research work on histone metabolism in the context of DNA damage as an EMBO fellow at Cancer Research UK. There he was awarded the 2004 Kirsten-Hardiman Redon Prize for his paper (Cell, 115: 537-549) that "shows outstanding research novelty" and was ranked among the top 12 candidates for the 2004 European Cell Signaler Award. He then joined the faculty of the Department of Biomedical Sciences at the Florida State University College of Medicine. He was awarded the “Outstanding Junior Faculty Researcher 2009-2010” award from the Florida State University College of Medicine for his seminal work on regulated histone proteolysis (Nature Cell Biology, 11: 925-933). Dr. Gunjan has nearly two decades of experience in the field of chromatin structure and function, and well over a decade of experience in the field of DNA damage and repair. He continues to work on trying to understand how histones and chromatin maybe contributing to DNA repair, genome stability and cancer prevention.

Find out more

Sign up for the Andor Newsletter!

Receive articles like this one, product launches, press releases and more with our quarterly newsletter focusing on either Physical Science or Life Science. It's free to subscribe and you can opt out at any time.

NAME EMAIL

Physical ScienceLife ScienceSUBMIT