Super-Resolution Microscopy

An introduction to Super-Resolution

Resolution limits imposed by light diffraction have always been major obstacle in analysing microscopic images and obtaining insights into the fabric of living cells. To date, our best optical tools have only revealed structures measuring approximately 200 nm across and anything below that limit has been inaccessible for direct observations. Unfortunately, most cellular organelles involved in physiologically important processes involving cell-to-cell communication, growth and response to number of environmental signals are often below that limiting threshold of 200 nm. For example, synaptic vesicles, receptor proteins complexes and cellular skeletal assemblies can be as small as 50 nm yet our understanding of their function could be greatly improved if only direct optical recording was possible.

Several novel microscopic techniques made this possible and previously inaccessible nano-environments began to be mapped. PALMIRA, STORM, FIONA as well as a number of related approaches have started to yield outstanding results. For the first time direct optical recordings of sub-resolution structures allowed addressing important structural and physiological questions. Answers to these questions will dramatically improve our understanding of the nature of processes involving responses of living cells to stress, damage, proliferation and interactions in their complex physiological milieu.

Multimedia Library
Application Images (10)
Publications Database
Point spread function optimization for STORM using adaptive optics
Click chemistry for the conservation of cellular structures and fluorescent proteins: ClickOx
Multiple Spatial and Kinetic Subpopulations of CaMKII in Spines and Dendrites as Resolved by Single-Molecule Tracking PALM
Micromirror structured illumination microscope for high-speed in vivo drosophila brain imaging
Quantitative single-molecule localization microscopy combined with rule-based modeling reveals ligand-induced TNF-R1 reorganization toward higher-order …
A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment
TestSTORM: Simulator for optimizing sample labeling and image acquisition in localization based super-resolution microscopy
Data Analysis for Single-Molecule Localization Microscopy
Focus on Super-Resolution Imaging with Direct Stochastic Optical Reconstruction Microscopy (dSTORM)
Extracellular Monomeric Tau is Sufficient to Initiate the Spread of Tau Pathology
Ezrin Tunes the Magnitude of Humoral Immunity
Superresolution Imaging with Standard Fluorescent Probes
Correlative live-cell and superresolution microscopy reveals cargo transport dynamics at microtubule intersections
Nanoscopy with more than 100,000'doughnuts'
Super Resolution Microscopy Reveals that Caveolin-1 Is Required for Spatial Organization of CRFB1 and Subsequent Antiviral Signaling in Zebrafish
Nanoscopy with more than 100,000 'doughnuts'
Optical Photon Reassignment Microscopy (OPRA)
Simple super-resolution live-cell imaging based on diffusion-assisted Forster resonance energy transfer