The Comet Assay

A Versatile and Sensitive Technique for the Assessment of DNA Damage and Repair

The Comet Assay is a powerful tool for the assessment of DNA integrity with applications as diverse as cancer research, safety testing of pharmaceuticals and chemicals, environmental and occupational studies, dietary and even fertility research. Because the assay can be adapted to virtually any cell-type from almost any organism, study designs can be created to characterize DNA damage and repair as well as DNA integrity and applied to both the impact of treatments and exposures as well as to endogenous factors. In this webinar two well-known scientists in this field describe recent research applications the comet assay.

Prof. Diana Anderson (University of Bradford): Prof. Anderson and her research team examined differences in the sensitivity to genomic damage of lymphocytes harvested from cancer patients, pre/suspect cancer patients and normal healthy volunteers. They developed a technique in which the cells were exposed to ultra-violet radiation (UVA) and assessed for the resulting levels of DNA damage using the comet assay. They discovered that genomic damage in lymphocytes from cancer patient samples plateaued and did not decrease as UVA intensity decreased. In contrast, lymphocyte response patterns for healthy individuals returned towards control values as UVA intensity decreased. Responses in samples from pre/suspected cancers patients showed intermediate results. Results indicated that characterisation of differences in lymphocyte sensitivity to UVA enabled discrimination between cancer patients, pre/suspect cancer patients and healthy volunteers. This relationship could be used in an assay that functions as a stand-alone test or as a possible adjunct to other tests for cancer diagnosis or screening.

Marie Vasquez (Helix3): At Helix3 we develop new methods and techniques to enhance the efficacy and expand the applicability of safety testing for regulatory submissions. Every day, new drugs, chemicals, medical treatments and consumer products are developed to be more specific, sensitive, and/or complex than their predecessors. Our goal is to develop and conduct GLP research that will help regulators and the industry to reliably assess the genotoxic risk with greater confidence and sensitivity. Our work with different applications of the comet assay including in vivo, acellular, and clinical exposures has helped to identify the cause/mechanism for equivocal or positive in vitro genotoxicity tests. This has led to the re-formulation of less harmful products and the confident progression of good products that might have otherwise been discontinued.

Dr. Mark Browne (Andor Technology): Since its inception in the 1980’s, the Comet Assay rapidly evolved as a valuable tool for research and safety testing. The adoption of the OECD Guidelines “In-Vivo Mammalian Alkaline Comet Assay” (TG489) on September 26th 2014, marks a significant milestone as the assay is now officially embraced for safety testing among regulators responsible for human health and well-being. To mark this important development, Andor Technology is delighted to announce Komet 7 and Komet 7GLP the latest evolutions of Komet solutions first offered by Kinetic Imaging in 1992.

Komet 7 and Komet 7GLP embrace new OECD requirements building on the experience developed over the last 20 years. Komet 7 and 7GLP maintain backward compatibility for hundreds of existing users, while also enabling new possibilities using the latest cameras, light sources and computer systems. Komet 7, now validated with Windows 7, is the most highly referenced comet assay analysis solution in research publications (PubMed and Google Scholar) and has found widespread adoption in research and safety testing. Key features of the new software will be presented.

Multimedia Library
Application Images (8)
Publications Database
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
CDC42 GTPase Activation Affects HeLa Cell DNA Repair and Proliferation Following UV Radiation‐Induced Genotoxic Stress
TGFβ1 Protects cells from γ-IR by enhancing the activity of the NHEJ repair pathway
Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays
Activation of DNA damage repair pathways in response to nitrogen mustard-induced DNA damage and toxicity in skin keratinocytes
Influence of oxidative stress, diaphragm fatigue, and
Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects
Catalytic antioxidant AEOL 10150 treatment ameliorates sulfur mustard analog 2-chloroethyl ethyl sulfide-associated cutaneous toxic effects
Effects of seven chemicals on DNA damage in the rat urinary bladder: A comet assay study
Mitogen-activated protein kinase signal transduction and DNA repair network are involved in aluminum-induced DNA damage and adaptive response in root …
Sodium arsenite induced changes in survival, growth, metamorphosis and genotoxicity in the Indian cricket frog (< i> Rana limnocharis</i>)
Hypoxia Provokes Base Excision Repair Changes and a Repair-Deficient, Mutator Phenotype in Colorectal Cancer Cells
Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay
Effects of Biovar I and Biovar II of< i> Ureaplasma urealyticum</i> on Sperm Parameters, Lipid Peroxidation, and Deoxyribonucleic Acid Damage in Male Infertility
MALDI-TOF fingerprinting of seminal plasma lipids in the study of human male infertility
Genotoxicity analysis of cerium oxide micro and nanoparticles in Wistar rats after 28 days of repeated oral administration
Sodium Fluoride Promotes Apoptosis by Generation of Reactive Oxygen Species in Human Lymphocytes
Genotoxicity assessment of cerium oxide nanoparticles in female Wistar rats after acute oral exposure
The differential localization of a methyl group confers to two triterpenes present in the olives a different anti-breast cancer activity.

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