The ultimate limit to analytical sensitivity is reliable single molecule detection. Recent technical advances in optical detection and manipulation have made the detection of isolated, light emitting probe molecules a reality. Thus we are witnessing a burgeoning interest in the imaging and spectroscopy of single molecules, particularly within the fields of cell biology and drug discovery.
Of particular importance to biology is the possibility of direct, real-time visualization of single biological macromolecules and their assemblies under native physiological conditions, offering great promise for enhancing our understanding of the behavior, interactions, mechanisms and trafficking of individual biological macromolecules within the living cell.
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1. iXon helps in discovering secrets behind protein unfolding
2. Sliding and Jumping of a EcoRV DNA-binding Enzyme – A Single Molecule Mechanistic Study
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Providing a cross-section of publications arising from laboratories using Andor's EMCCD technology for single molecule detection.
Single molecule imaging and FLIM show different structures for high and low-affinity EGFRs in A431 cells
Marisa L. Martin-Fernandez et al., Biophysical Journal 94:803-819 (2008)
Dissecting the multi-step reaction pathway of an RNA enzyme by single-molecule kinetic fingerprinting
S. Liu, G. Bokinsky, N. G. Walter, X. Zhuang, Proc. Natl. Acad. Sci. USA 104, 12634-12639 (2007)
Two Distinct Binding Modes of a Protein Cofactor with its Target RNA
G. Bokinsky, L. G. Nivón, S. Liu, G. Chai, M. Hong, K. M. Weeks, X. Zhuang, J. Mol. Biol. 361, 771–784 (2006)
Andor's EMCCD is the ideal detector technology for dynamic single molecule imaging. The extraordinary Signal to Noise (S/N) enhancement offered is significantly greater than that afforded by conventional CCD cameras operated at fast readout speeds. EMCCDs exhibit frame rates that are ideally suited to dynamic acquisition of transient single molecules and their interactions.More Details...
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