Spectral Flow Cytometry is a technique based on conventional Flow Cytometry where a spectrograph and multichannel detector (usually CCD) is substituted for the traditional mirrors, optical filters and photomultiplier tubes (PMT) in conventional systems. Flow Cytometry allows for rapid multiparameter collection and analysis of data on single cells or particles.
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1. Raman Spectral Flow Cytometry (RSFC)
A key and continuing goal in the development of flow cytometry techniques is the ability to measure ever more parameters for each particle under test. Work carried out by Prof John Nolan’s group at La Jolla Bioengineering Institute, and reported recently by Watson et. al. in Cytometry A,outlines the development and operation of a Raman Spectral Flow Cytometer (RSFC), which is perhaps the most radical and challenging approach to current efforts in spectral flow cytometry.
A Flow Cytometer for the Measurement of Raman Spectra
Dakota A Watson, Leif O Brown, Danial F Gaskill, Mark Naivar, Steven W Graves, Stephen K Doorn and John P Nolan, Cytometry Part A, Vol 73A, p119-128, (2008)
Cellular discrimination based on spectral analysis of intrinsic fluorescence
Gregory R Goddard, J P Houston, John C Martin, Steven W Graves, James P Freyer, Proc. Of SPIE, Vol. 6859 685908-1 (2008)
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