Resolution limits imposed by light diffraction have always been major obstacle in analysing microscopic images and obtaining insights into the fabric of living cells. To date, our best optical tools have only revealed structures measuring approximately 200 nm across and anything below that limit has been inaccessible for direct observations. Unfortunately, most cellular organelles involved in physiologically important processes involving cell-to-cell communication, growth and response to number of environmental signals are often below that limiting threshold of 200 nm.
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1. Whole Cell 3D Super-Resolution Imaging by ‘STORM’ Employing Back-illuminated EMCCD Detection
2. Novel nanoscopy technique – imaging organelles at 40 nm resolution with PALMIRA
3. Novel insights into cell nucleus – subdiffraction imaging of nuclear pores
4. An unlikely connection – double-helix and super-resolution
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Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
Schermelleh et al., Science 320, (2008) Jun 6; 320 (5881):1332-6.
Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
Zhuang et al., Science 319, (2008) Feb 8; 319 (5864):810-3
Andor's EMCCD is the ideal detector technology for super-resolution imaging. The extraordinary Signal to Noise (S/N) enhancement offered is significantly greater than that afforded by conventional CCD cameras operated at fast readout speeds. EMCCDs exhibit frame rates that are ideally suited to dynamic acquisition of transient, single molecular events occurring during reversible fluorophore transitions in specimen.More Details...
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