Andor

Dragonfly

The most complete imaging solution ever!

The game-changer in confocal microscopy - with the Andor Dragonfly you can image at an unrivalled, multi-modal combination of speed, sensitivity and confocality.

WATCH THE DRAGONFLY STORY

It's more than confocal.

The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and thick tissue imaging all benefit from the system’s speed and sensitivity. Dragonfly delivers real-time visualization for rapid specimen evaluation and GPU-accelerated deconvolution to maximize resolution and throughput.

  • Instant Confocal
  • Simultaneous multi-color TIRF
  • Laser widefield imaging
  • Single molecule imaging
  • VIS - NIR wavelengths
Fast Acquisition Unibody Design Self Adjusting Low Phototoxicity
 

Fast 2D - 6D imaging

The Dragonfly offers you a sharp insight into the world of your specimen by employing a novel multi-point confocal system, optimized for fast, flexible and sensitive scanning. It extends your sample’s longevity and significantly speeds up data acquisition.

As well as confocal, the system provides further extraordinary control of illumination by offering enhanced widefield and TIRF microscopy, extending the reach from nm to mm and from single molecules to cells, tissues, organoids and embryos.

Traditional Point Scanning Confocal

Dragonfly Confocal Imaging System

Laser Range Confocal Speed Aperture Camera Zoom Illumination Zoom Pinholes Ø PDF
400 - 800 nm 400 fps 22 mm 1x  |  1.5x  |  2x 1x  |  2x  |  4x  |  6x 40 µm  |  25 µm

Dragonfly captures in 16-bit dynamic range, therefore can obtain both low and high signal detail within a single capture.

Optical excellence for all experiments

Andor Dragonfly features multiple innovative optical solutions to create the best possible combination of light uniformity, throughput, resolution and power in a confocal microscopy system.

Borealis Illumination
Magnification
Power Density



Borealis Uniform Illumination

Uniformity of illumination plays a significant role in obtaining
seamless image tiling and more accurate cross-field analysis.

With Borealis, and its unique Perfect Illumination Delivery™
system, you can ensure that the edges and corners of
your sample never suffer from non-uniformities.

Bacteria to organoids: an imaging platform for all

Confocal, widefield and TIRFM modes for your imaging experiment!

With three modalities at your command, you can explore the in vitro and in vivo biology of many different specimens, selecting the best model organism and modality to suit your needs. Imaging everything from organoids and deep neural structures along the z-dimension to yeast, bacteria and single molecules requires flexibility of imaging mode, magnification and illumination. This level of control preserves specimens and trades speed and longevity in dynamic and large scale imaging tasks.

Please select your preferred illumination method below to see how Dragonfly meets your requirements.

Multi-Point Confocal

At the heart of the Dragonfly Imaging Platform is a newly designed microlens confocal scanner, tightly integrated with our highest sensitivity cameras and presented via our new imaging software, Fusion. The result is speeds of 10 to 20 times faster than a traditional confocal, leaving you with no more waiting for an image to be built up point-by-point and line-by-line. Dragonfly offers the following major benefits:

  • Minimal phototoxicity and photobleaching - ideal for live or delicate specimens.
  • High speeds for imaging fast dynamic events, or high throughput.
  • Large uniform field of view for larger specimens or montage stitching.

Simplify experimental workflow

Central to the Dragonfly concept is the goal of maximizing throughput, which is achieved by optimizing both imaging performance and data flow. Fusion and Imaris provide seamless transitions from imaging to deconvolution, visualization to analysis. As a result you spend more time collecting high quality data and less time pre-processing and transferring between systems.

Speed Hypothesis Testing

Hypothesis testing is a fundamental part of the scientific method. The familiar cycle of data creation and evaluation are delivered by the Dragonfly workflow, supporting demanding experimental regimes. Extended observations, large fields of view and high quality data provide the input for powerful statistical analysis and data mining.

Experiment and data management

 
 

Fusion is the result of over 100 person-years of shared imaging and development experience, making it easy to design an experiment, choose necessary devices and configure optimal data acquisition protocols. It simplifies the control of the Dragonfly system, translating imaging modes into software states and fluorophores into channels to help you build protocols in just a few mouse clicks.

Integrating deconvolution is simple, tasking the GPU with the associated calculations so that acquisition continues unaffected: a big time saver! Results are delivered with your data, all in one software package. Real-time 3D visualization, split channel view and MIP are fully configurable during acquisition to let you view the data in a mode which best suits your experiment.

Real-Time Render
Protocol Manager
Deconvolution



Real-Time Rendering

Real-time rendering in Fusion allows for an on-going verification of the quality of your experiment and the status of your sample.

Having a near-instantaneous feedback on the 3D properties of the specimen is critical for evaluation of experimental parameters including signal to noise, illumination power and sample viability, its position in 3D and in relation to its cellular or tissue neighbours. Render quality and speed can be rapidly adjusted following any change in your experiment and can be easily accessed in the Fusion software control panel.

Analyse, interpret, assess, reiterate

All the necessary functionality for data management, visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets with Imaris

Visualize Complex Data
Trace Filaments
Analyse and Understand Cell Fate



 

Visualize Complex Data

Welcome to a new era of data interpretation, plotting and presentation. ImarisVantage enables you to compare and contrast experimental groups by visualizing your image data in five dimensions as uni- or multi-variate scatterplots. Along with the use of box + whisker plots Vantage will help you interpret intrinsically complex and dynamic phenomena.

ImarisVantage allows researchers to dissect their multi-dimensional, multi-object images by creating a series of fully customizable plots for better understanding of hidden relationships and associations between calculated measurements, objects or groups of objects.

Additional Resources

The Andor Learning Center hosts a wide range of tutorial videos, technical articles and webinars to guide you through the range of products for all your imaging needs. We have provided some links below which will get you started on some of our most recent uploads.