Andor Dragonfly - High Speed Confocal Microscopy | Andor


The most complete imaging solution ever... just got better!

The game-changer in confocal microscopy. With the Andor Dragonfly, you can image at an unrivalled combination of speed, sensitivity, confocality and resolution beyond the diffraction limit. Now available in 200 series and 500 series.


It's more than confocal...

The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and thick tissue imaging all benefit from the system’s speed and sensitivity. Dragonfly delivers real-time visualization for rapid specimen evaluation and ClearView-GPU™ deconvolution to maximize resolution and throughput.

  • Instant Confocal
  • Simultaneous dual-colour TIRF
  • Laser widefield imaging
  • Single molecule imaging
  • VIS - NIR wavelengths
Fast Acquisition 3D Visualisation 3D Visualisation Self Adjusting Low Phototoxicity

Fast 2D - 6D imaging

The Dragonfly offers you a sharp insight into the world of your specimen by employing a novel multi-point confocal system, optimized for fast, flexible and sensitive scanning. It extends your sample’s longevity and significantly speeds up data acquisition.

As well as confocal, the system provides further extraordinary control of illumination by offering enhanced widefield and TIRF microscopy, extending the reach from nm to mm and from single molecules to cells, tissues, organoids and embryos.

Traditional Point Scanning Confocal

Dragonfly Confocal Imaging System

Laser Range Confocal Speed Aperture Camera Zoom Illumination Zoom Pinholes Ø PDF
400 - 800 nm 400 fps 22 mm 1x  |  1.5x  |  2x 1x  |  2x  |  4x  |  6x 40 µm  |  25 µm

Dragonfly captures in 16-bit dynamic range, therefore can obtain both low and high signal detail within a single capture.

Dragonfly Models

Dragonfly 200
Dragonfly 500

Dragonfly is now available in a more compact package, the 200 series, suitable for both inverted and upright microscopes.

The 200 series offers many of the key features that make Dragonfly performance exceptional: Confocal speeds of up to 400 fps; Borealis™ enhanced illumination for a large and flat field of view; ClearView-GPU™ deconvolution; dual-camera acquisition; choice of optical zoom and pinhole size.

For those who might be considering a new or additional confocal, but looking for something that pushes new imaging boundaries. The Dragonfly 200 offers a price-point comparable to traditional point scanning technology.


The combination of Andor’s Dragonfly, iXon EMCCD cameras and Fusion software provide unparalleled access to super-resolution techniques to image beyond the diffraction limit of optical microscopy. Expand your research from single molecules across six orders of magnitude to whole organisms. All on one system! Choose from our latest camera-based super-resolution technique, SRRF-Stream; iterative ClearView-GPU™ deconvolution or the localisation technique known as dSTORM. Each delivers a different and unique view into the nanometer world.



Deconvolution is an invaluable tool for bringing out the subtle details of a 3D data set that might otherwise go unnoticed, even in a good quality confocal stack. Attain a lateral resolution down to 140nm and axial resolution to 250nm.

ClearView-GPU™ deconvolution has been made an integral part of the system to enhance the finest features of your image. Up to 50x faster than CPU methods, GPU-acceleration and processing while acquiring allow immediate visualisation and comparison with the original data! Use deconvolution for the highest 3D resolution, especially powerful as the only super-resolution tool that can be applied to high-speed imaging such as live-cell and multi-dimensional imaging.


Why Your Next Confocal Should Be Dragonfly

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Dragonfly is a high performance multi-modal imaging platform. In this article, we focus on Dragonfly’s multi-point scanning confocal imaging performance and compare it to single point scanning, which has become the dominant technology over the last 30 years.

We show that Dragonfly exceeds or matches the performance of point scanners in all important aspects. As life science research accelerates and demands greater throughput for deeper study, we suggest the community should consider this new and powerful platform wherever there is a need for fast, sensitive, high resolution confocal imaging.

Discover how Dragonfly excels in:

  • Productivity
  • Speed
  • Sensitivity
  • Extended spectral range
  • Quantifiable results
  • Imaging thick specimens

Bacteria to organoids: an imaging platform for all

Confocal, widefield and TIRFM modes for your imaging experiment!

With three modalities at your command, you can explore the in vitro and in vivo biology of many different specimens, selecting the best model organism and modality to suit your needs. Imaging everything from organoids and deep neural structures along the z-dimension to yeast, bacteria and single molecules requires flexibility of imaging mode, magnification and illumination. This level of control preserves specimens and trades speed and longevity in dynamic and large scale imaging tasks.

Please select your preferred illumination method below to see how Dragonfly meets your requirements.

Multi-Point Confocal

At the heart of the Dragonfly Imaging Platform is a newly designed microlens confocal scanner, tightly integrated with our highest sensitivity cameras and presented via our new imaging software, Fusion. The result is speeds of 10 to 20 times faster than a traditional confocal, leaving you with no more waiting for an image to be built up point-by-point and line-by-line. Dragonfly offers the following major benefits:

  • Minimal phototoxicity and photobleaching - ideal for live or delicate specimens.
  • High speeds for imaging fast dynamic events, or high throughput.
  • Large uniform field of view for larger specimens or montage stitching.

Optical excellence for all experiments

Andor Dragonfly features multiple innovative optical solutions to create the best possible combination of light uniformity, throughput, resolution and power in a confocal microscopy system.

Borealis Illumination
Power Density

Borealis Uniform Illumination

Uniformity of illumination plays a significant role in obtaining
seamless image tiling and more accurate cross-field analysis.

With Borealis, and its unique Perfect Illumination Delivery™
system, you can ensure that the edges and corners of
your sample never suffer from non-uniformities.

Experiment and data management


Fusion is the result of over 100 person-years of shared imaging and development experience, making it easy to design an experiment, choose necessary devices and configure optimal data acquisition protocols. It simplifies the control of the Dragonfly system, translating imaging modes into software states and fluorophores into channels to help you build protocols in just a few mouse clicks.

Integrating deconvolution is simple, tasking the GPU with the associated calculations so that acquisition continues unaffected: a big time saver! Results are delivered with your data, all in one software package. Real-time 3D visualization, split channel view and MIP are fully configurable during acquisition to let you view the data in a mode which best suits your experiment.

Real-Time Render
Protocol Manager
Image Processing

Real-Time Rendering

Real-time rendering in Fusion allows for an on-going verification of the quality of your experiment and the status of your sample.

Having a near-instantaneous feedback on the 3D properties of the specimen is critical for evaluation of experimental parameters including signal to noise, illumination power and sample viability, its position in 3D and in relation to its cellular or tissue neighbours. Render quality and speed can be rapidly adjusted following any change in your experiment and can be easily accessed in the Fusion software control panel.

Simplify experimental workflow

Central to the Dragonfly concept is the goal of maximizing throughput, which is achieved by optimizing both imaging performance and data flow. Fusion and Imaris provide seamless transitions from imaging to deconvolution, visualization to analysis. As a result you spend more time collecting high quality data and less time pre-processing and transferring between systems.

Speed Hypothesis Testing

Hypothesis testing is a fundamental part of the scientific method. The familiar cycle of data creation and evaluation are delivered by the Dragonfly workflow, supporting demanding experimental regimes. Extended observations, large fields of view and high quality data provide the input for powerful statistical analysis and data mining.

Analyse, interpret, assess, reiterate

All the necessary functionality for data management, visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets with Imaris

Visualize Complex Data
Trace Filaments
Analyse and Understand Cell Fate


Visualize Complex Data

Welcome to a new era of data interpretation, plotting and presentation. ImarisVantage enables you to compare and contrast experimental groups by visualizing your image data in five dimensions as uni- or multi-variate scatterplots. Along with the use of box + whisker plots Vantage will help you interpret intrinsically complex and dynamic phenomena.

ImarisVantage allows researchers to dissect their multi-dimensional, multi-object images by creating a series of fully customizable plots for better understanding of hidden relationships and associations between calculated measurements, objects or groups of objects.

Additional Resources

The Andor Learning Center hosts a wide range of tutorial videos, technical articles and webinars to guide you through the range of products for all your imaging needs. We have provided some links below which will get you started on some of our most recent uploads.