Photostimulation

Photostimulation (PS) describes a rapidly evolving range of optical techniques with an increasing impact on scientific enquiry and experimentation. All PS techniques target optical radiation at user-defined region(s) of the specimen and use optical imaging to observe, and often to measure, the impact of the “perturbation”.

ModelDescriptionBuy it Now...
Mosaic
Simultaneously illuminate multiple regions of interest.
MicroPoint
Diffraction limited illumination from a pulsed dye-laser.
FRAPPA
Unique design for galvo-scanned photobleaching and activation.


Photostimulation

Photostimulation describes a rapidly evolving range of optical techniques with an increasing impact on scientific enquiry and experimentation. PS has developed over the last two decades alongside the revolution of fluorescent proteins in biology (ref 7, 8), the instrumental and technological developments of confocal laser scanning microscopy (CLSM), solid state light sources (lasers and LEDs), fast galvo and optical MEMS technology, and of course the ubiquitous personal computer. We include among these techniques:

  • Photoactivation and switching
  • Constrained or adaptive illumination
  • FRAP
  • Ablation, cutting and marking
  • Uncaging of caged compounds
  • Optogenetics e.g. channelrhodopsin2
Specification Sheets




Product Portfolio

Multimedia Library
Application Images (6)
Application Movies (16)
Publications Database
Anisotropic stress orients remodelling of mammalian limb bud ectoderm
An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms
Gap geometry dictates epithelial closure efficiency
The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function
Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity
Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis
Neuronal serotonin release triggers the heat shock response in C. elegans in the absence of temperature increase
In situ biosynthesis of fluorescent platinum nanoclusters: Towards self-bio-imaging guided cancer theranostics
Andor Mosaic 3: Multilevel Modulation of a Sensory Motor Circuit during Sleep and Arousal
Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension
Directional migration of leading-edge mesoderm generates physical forces: Implication in Xenopus notochord formation during gastrulation
Tissue damage detection by osmotic surveillance
Strongly alkaline pH avoidance mediated by ASH sensory neurons in< i> C. elegans</i>
Compartmentalized Calcium Transients Trigger Dendrite Pruning in Drosophila Sensory Neurons
Wounded cells drive rapid epidermal repair in the early Drosophila embryo
Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii
Rapid Fluorescence Switching by Using a Fast Photochromic [2.2] Paracyclophane-Bridged Imidazole Dimer
Severing and end-to-end annealing of neurofilaments in neurons
In vivo Laser Axotomy in C. elegans
Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration