FRAPPA

Andor’s FRAPPA uses a dual galvanometer scan head to provide a computer-steered laser beam delivery system. It can be configured in line with a CSU and/or camera and operates in two modes CSU By-Pass Mode and Frap Mode.

  • "Bypass" mode provides 1:1 relay imaging for "in-line" configuration
  • "FRAPPA" mode performs laser scanning via imaging C-port
  • Diffraction limited spot size ~0.6 μm @488 nm FWHM
  • "In-line" operation enables use of all laser lines for FRAPPA actions
  • Mode switching in < 10 ms

FRAPPA

Share all laser lines for photo stimulation and imaging

FRAPPA is a galvo scanning instrument, named by conjoining acronyms for fluorescence recovery after photobleaching (FRAP) and photoactivation (PA). FRAPPA has a unique switching design that allows it to be configured in the imaging path. In bypass mode it acts as a relay optic, projecting an image to the detector, while in scanning mode it acts as a laser scanner, targeting user-defined regions of the specimen. This "in-line" configuration allows it to utilize the same wavelength for imaging and photo-stimulation.

The cost of "in-line" operation is sequential execution of imaging and photostimulation, but the switching speed is optimized at ~10 ms to minimize its impact. FRAPPA utilizes galvo technology, has a single laser input and is designed to deliver a diffraction limited spot from CW and pulsed lasers in the wavelength range 400-800 nm.

These features make it an attractive option for photobleaching, switching and activation as well as DNA damage studies.

Specifications Summary
Transmission 400 to 800 nm
Resolvable spot size Near diffraction limited
Laser inpute Single mode fiber FC
Beam power Up to 2 Watts optical
Intensity control AOTF 0.1 to 100%
Laser compatibility Pulsed or CW
Pixel dwell time (minimum) 20 μs
Certification CDRH IIIb
Features & Benefits
"Bypass" mode provides 1:1 relay imaging for "in-line" configuration
"FRAPPA" mode performs laser scanning via imaging C-port
Diffraction limited spot size ~0.6 μm @488 nm FWHM
"In-line" operation enables use of all laser lines for FRAPPA actions
Mode switching in < 10 ms
Integrated control with iQ2 software provides "point-and-shoot" and protocol modes
Arbitrary multi-region scanning of points, rectangles and polygons
Share imaging lasers with Andor's unique multi-port adapter
Active blanking output for ALC integration
Product Components
FRAPPA is available in a single model for "in-line" or dichroic configuration. With C-mount input and output, FRAPPA is easy to configure with the "in-line" setup, FRAPPA uses the bypass mode for imaging and the scanning mode for photo-stimulation. Its output can be coupled directly to a camera or a confocal scanner e.g. CSUX.
FRAPPA can be used for simultaneous photo-stimulation and imaging when configured on a separate microscope C-port and used with a dichroic mirror. FRAPPA is compatible with visible to near IR lasers operating in CW or pulsed mode (400 - 800 nm) for photostimulation.
Left: In bypass mode the FRAPPA acts as a 1:1 relay imaging system. As shown by the orange beam path the X galvo mirror is positioned at the instrument pupil and completes a 4f imaging path..
Right: In FRAPPA mode, the laser beam is injected from the laser input port and is steered to the Y galvo. The X galvo is positioned to scan the laser beam into the microscope to execute the defined photo-stimulation.
Multimedia Library
Application Images (2)
Application Movies (2)