MicroPoint

MicroPoint provides a flexible and field-proven tool for photo-stimulation. Supplied with a patented compact, pulsed nitrogen pumped tunable dye laser it is capable of ablation, bleaching and uncaging over a wavelength range of 365 to 656 nm.

  • Simultaneous laser delivery, microscope viewing and image acquisition
  • Low maintenance with fiber optic delivery that maintains alignment when system is moved
  • Quick set-up with manual beam positioning or automatic pattern generation
  • User control of ablation and illumination plane provided by z-axis telescope
  • Precise control of energy provided by motorized variable attenuator slide

MicroPoint

Simultaneous and precise illumination and ablation

MicroPoint provides a flexible and field-proven tool for photo-stimulation. Supplied with a patented compact, pulsed nitrogen pumped tunable dye laser it is capable of ablation, bleaching and uncaging over a wavelength range of 365 to 656 nm. Broad wavelength range and energy control allow MicroPoint to be optimized for a wide range of scenarios. More than 20 wavelengths can be utilized with available dye resonator cells, while appropriate dichroic filter sets enable simultaneous imaging and photo-stimulation of the specimen. MicroPoint is supplied with a UV-Vis imaging quality Epi illumination adapter for both current and previous generation microscopes from Leica, Nikon, Olympus and Zeiss.

MicroPoint systems consist of a wavelength tuneable pulsed laser, coupling optics, beam steering optics, a microscope adapter, a selection of beam splitters and interference filters, and a motorized or manually driven optical attenuator to adjust spot size and power. There are two MicroPoint versions available:

MicroPoint Manual

Angular and spatial alignment of the illumination at the sample target is manually controlled via a 2-axis joystick. This manual version can be upgraded in-situ to provide galvo or bluetooth control.

MicroPoint Galvo

Galvanometer based beam steering is provided through PC control, enables precise and repeatable laser ablation and/or illumination in synchronization with other experiment parameters.

Specifications Summary
Transmission 365 nm to 656 nm
Spectral bandwidth 4 nm FWHM
Transmission variation 0.1%-100%
Resolvable spot size Near diffraction limited
Average power 750 μW, 15 Hz / 50 μJ
Peak power 12 kW
Pulse width 3 to 5 nsec
Pulse repetition rate 0 to 15Hz
Certification CDRH IIIb
Features & Benefits
Simultaneous laser delivery, microscope viewing and image acquisition
Low maintenance with fiber optic delivery that maintains alignment when system is moved
Quick set-up with manual beam positioning or automatic pattern generation
User control of ablation and illumination plane provided by z-axis telescope
Precise control of energy provided by motorized variable attenuator slide
Multimedia Library
Application Images (1)
Application Movies (8)