Mosaic

Mosaic is the perfect tool for active illumination applications such as Optogenetics, optophysiology, photobleaching/activation and uncaging. This patented combination of digital mirror device used with a microscope delivers high speed targeting of complex light patterns to your sample. For optogenetics you can even combine complimentary activating and inhibitory wavelengths at the same time for tight spatial target protein control.

  • Unlimited flexibility in shape, size, complexity of illumination mask
  • Simultaneous illumination of multiple regions of interest
  • Precise illumination of areas of interest that protects the target specimen
  • Zero delta acquisition time for true digital excitation
  • Complementary illumination option enables on and off control

Mosaic Infinity

Optogenetic Specialist

Optogenetics is a rapidly growing technique used across an ever broadening array of research fields. Investigators now genetically modify many signalling pathway elements to enable precise and spatially targeted light control of biology down to a single cell or sub-cellular level. Mosaic’s original and patented combination of DMD (micro-mirror array) with microscopy delivers the perfect tool for optophysiology.

Neuroscience, probably the largest research field using targeted light, commonly uses an upright microscope. Mosaic Infinity has been designed to couple simply to most upright scopes

  • Flexible patterning for complex networks and dynamics
  • High-speed (5KHz) to meet biological demands
  • Simultaneous dual-colour illumination (excite & inhibit)
  • Supports several light source types (e.g. laser, LED, Hg)
  • Can be used with p-CLAMP™
  • Also capable of uncaging and photo-bleaching/activation

Mosaic & Mosaic Duet

Simultaneous illumination of multiple regions of interest in real time and with zero delta acquisition time

Mosaic is a patented instrument platform built around MEMS Digital Mirror Devices (DMD). DMDs were developed at Texas Instruments in 1987 and are now in widespread use in digital projectors and other display devices. The DMD comprises an array of individually addressable micro-mirrors that can be switched "on and off" (tilted) with MEMS "hinge" elements. DMD arrays contain hundreds of thousands to millions of micro-mirrors.

Mosaic exploits DMD in a proprietary programmable platform, integrated with scientific light sources including lasers, LEDs and arc lamps, and operates from 360 to 800 nm. It is offered with a range of high performance microscope adapter optics and can be integrated with CLSM, spinning disk and wide field imaging modalities.

High speed frame switching (5000 Hz) makes Mosaic suitable for many dynamic applications including bleaching, uncaging, photoswitching, optogenetics and constrained illumination. Variable intensity distributions can be achieved by rapid gating of mirror patterns.

Mosaic has a unique capability to illuminate in parallel an arbitrary number of complex regions (sometimes called “zero delta t”) that sets it apart from galvo-based devices and makes it especially attractive for uncaging, photoswitching and light activation. It is a unique tool for the study of optically stimulated intra and inter-cellular activity in neuroscience and physiology, as well as for function-structure studies with photoswitching fluorescent proteins. Mosaic uses dichroic coupling to the microscope light path and is therefore capable of simultaneous stimulation and imaging.

Features & Benefits
Unlimited flexibility in shape, size, complexity of illumination mask
Simultaneous illumination of multiple regions of interest
Precise illumination of areas of interest that protects target specimen and fluorophore
Zero delta acquisition time for true digital excitation
Complementary illumination option enables on and off control in optogenetics studies
Longest lifetime and lowest maintenance with rugged semiconductor device
Installation and training available, ensuring optimal setup and faster familiarization
Filter sets available for Channel rhodopsin, Halorhodopsin, csChrimson, and more
On-board memory of patterns and sequences for hardware triggered (software independent) pattern control (e.g.pCLAMP™).
Region specific grey-scaling available for light dosing and fine control of photostimulation
Simultaneous, asynchronous, region-defined imaging with photo-stimulation. A powerful tool when the same excitation wavelength is required.
Supporting software includes Andor iQ, Metamorph, Micro-Manager, and NIS Elements (region-dependent availability).
A well-documented SDK is available for those who wish to self-integrate.
Specifications Summary
Transmission 360 nm to 800 nm
Extinction ratio > 800:1
Optical pixel rise / fall time 2 µs
Minimum exposure time 50 μs – Software or External Expose, Sequence (AllOperational modes)
200 μs – Overlap mode (Software or External Expose)
200 μs – External Bulb Mode
Maximum frame rate 5000 fps (Mask Memory Mode – Overlap Mode)
Compliance CE Marked
RoHS Compliant
IEC 60825-1
FDA/ CDRH 21 CFR 1040.10
Multimedia Library
Application Images (4)
Application Movies (9)