Revolution DSD2

The Andor Revolution DSD2 is a simple confocal device delivering extraordinary imaging performance. Its simplicity lies in a compact patented optical design and laser-free operation, providing ease of retrofit to an existing fluorescence microscope, or an easy addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities, or if you run a core facility, you can increase your capacity and offer a lower cost solution with a DSD2. The ease of use is such that you will be imaging in no time, with minimal training required

  • Simple add-on confocal
  • Stunning image quality
  • Large field of view
  • Low running costs (laser-free)
  • Handles broad sample range

Revolution® DSD2

Flexibility

The simple design of the DSD2 means that it can be mounted to inverted and upright microscopes and fluorescence stereomicroscopes and macroscopes. With three confocal sectioning options, you can choose the optimal setting for your sample or magnification. This makes the DSD2 probably the most versatile confocal device on the market, addressing the broadest range of sample types. With no fixed laser wavelengths to limit you, you have freedom to choose the best wavelength choice for your research. Fixed or live, there is no limit to the possibilities.

Productivity

With a spinning disk at its core, the DSD2 naturally captures images at higher frame rates than a point scanning confocal. A three dimensional image, at a like-for-like resolution and comparable quality, can be captured in seconds on the DSD2 compared to minutes on a point scanning confocal. This offers two key benefits. Higher throughput of samples, so increasing productivity and earlier data review; and for live samples the ability to capture dynamics in higher temporal detail for greater insight.

Scaleability

The Revolution DSD2 can be used with additional Andor devices from our Photostimulation range. For example, add a MicroPoint to combine confocal imaging with ablation, such as used in damage & repair research, or add a Mosaic 3 for fast targeted illumination to use optogenetic models with DSD2. This simple device can in fact be used for sophisticated applications, now or as your research develops.

Quality

Combining structured illumination and spinning disk technologies with high sensitivity high dynamic range Andor sCMOS cameras, the Revolution DSD2 produces image quality that typically exceeds confocal images captured with laser point scanning systems. This latest generation builds on a field proven device, introducing even better performance and so even higher quality imaging.

Reliability

With no lasers required for the Revolution DSD2, and the simplicity of its design making it an easy device to align, there is no risk of owning a confocal device requiring regular attention to keep it operational. The benefit is worry free imaging for many years, and extremely low maintenance costs.

Features Benefits
Unique Disk Design Delivers images of high contrast with high dynamic range for outstanding results. Even with thick samples.
Confocal flexibility Three confocal sectioning modes to address all magnifications and provide freedom to select best match for sample
Large field of view Image large samples or larger numbers of cells to increase productivity
Broad spectrum, laser free 370 – 700nm excitation with full flexibility of filter choice
Easy alignment and low maintenance Minimal running costs for product longevity.
Optimized software and algorithms Up to 22 frames per second real-time confocal imaging for high productivity or live cell imaging. Intuitive control for fast operation and minimal training
Real-time control and viewing Switch viewing mode between widefield and confocal with one mouse click to ease locating sample
Confocal and Widefield modes Use DSD2 to image even weakly fluorescent samples which are difficult to see in confocal.
Microscope agnostic, including stereo and macroscopes Image using your preferred microscope according to your sample requirements
By-pass mode Remove the disk for higher speed fluorescence imaging

Innovation

The Revolution DSD2 is unique in its class in having 3 confocal settings, which we call High Signal, Medium Sectioning and High Sectioning. These settings will be chosen according to the objective magnification or sample type that you use. Typically you would expect to select High Sectioning for the most confocal image with the highest detail, as clearly demonstrated below in a sample captured at a low magnification. However, at higher magnifications and in thinner samples, you may find that to maximise on signal and sensitivity, you will need to select the High Signal setting. The choice is yours for you to capture the best quality images possible

A demonstration of the 3 settings at work on the DSD2 Images of the same sample taken using the three different disk sectors. Sample is the egg-chamber from Drosophila melanogaster at stage 10. Visible is the large oocyte with its tiny meiotic nucleus. Insets are a close-upof the nurse cells with large polyploid nuclei. You can see the improved resolution as the disk pattern moves from High signal to High sectioning. Stained are: DAPI (blue) to show the DNA, WGA-657 (green) to show membranes, primarily visible are nuclear membranes. Also stained by fluorescent in situ hybridization is the oskar mRNA that is enriched at the posterior cortex. Detection: Cy3-coupled tyramide amplification. Captured on a 25x (0.8NA) objective Courtesy of Dr Helena Jambor (of Tomancak Lab) at MPI Dresden.

Visualisation

In order to achieve the best quality results from the Revolution DSD2 and your research we strongly recommend combining Andor iQ with Bitplane Imaris.

The DSD2 will generate large data sets, especially in fields like developmental biology and Neuroscience. Imaris has the ability to view huge Gbyte data sets from the moment the data starts loading. You can then go on to use sophisticated visualisation and comprehensive analysis tools for multi-dimensional data sets, delivering greater insight to your research that you may not have thought possible

Key Specifications
Imaging 4 filter cube positions
Excitation range 370 – 700 nm
Emission range 410 – 750 nm
Frame rates (max) 22 fps (16-bit, confocal mode, full frame, 1x1 binning)
Confocality (PSF) 3 sectors provide 1.2 μm, 1.0 μm, 0.8 μm (FWHM)
Operating Principal Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample
Disk rotation speed 3000 rpm
Minimum Exposure 20 ms (1 full disk rotation)
Widefield By-pass Selectable mode completely removes disk from the imaging path
Switching time between disk sectors <3 s
Filter Cubes Turret 4 positions, user-replaceable cubes
Filter switching time <200 ms
Multimedia Library
Application Images (10)
Application Movies (6)
Product Photos (8)