Light sources are chosen for high brightness and efficient coupling to AI instruments. FRAPPA uses CW and pulsed lasers in the range 400-800 nm and powers up to 1W. MicroPoint is designed for pulsed dye-laser operation, but can use other lasers (on request) in the range 365-660 nm. Mosaic is compatible with lasers, arc lamps and super-luminous sources according to the application. Uncaging, photoactivation, photoswitching and optogenetics need lower power density so incoherent sources function well and are cost effective.
This high-power laser diode delivers a CV output of approximately 475 mW. The laser can be directly modulated at MHz rates, making it highly effective for both sustained and pulse mode operation. To ensure uniform illumination of Mosaic’s DMD, the optical fiber, which delivers the laser output into the Mosaic, is subjected to mode mixing or phase shearing. This homogenizes the optical intensity profile, achieving typical uniformity better than 5%.
ALC is an established market leader with up to 6 solid state laser lines in a single 19” rack enclosure and integral AOTF for rapid switching and blanking. The multi-port switching unit (MPU) provides unique flexibility with millisecond switching to one of three fiber ports for confocal, FRAPPA and/or TIRF.
The Lambda DG-4/DG-5 is a complete illumination system offering speed and versatility for experiments requiring rapid wavelength switching. The instrument retains all the advantages of interference filter based systems, yet eliminates temporal constraints imposed by traditional filter changing devices like filter wheels. Switching between any two wavelengths is achieved in less than 1.2 ms. This facilitates the ability to follow fast changes in ion concentrations in dual wavelength ratio imaging applications and to monitor changes in the studied system at additional wavelengths.
The AMH-200 is a 200 Watt DC stabilized metal halide light source with 2200 hours lamp life. Support from Andor iQ and 3rd party software make this a flexible and efficient source for a broad range of applications. The easily replaced lamp and liquid light guide provide broadband excitation (UV-Vis-NIR) with no alignment and offer outstanding uniformity. AMH-200- F6S includes a fast 6-position internal filter wheel providing a cost effective, integrated Epi illumination package.
Epi fluorescence microscopes have traditionally used Mercury or Xenon arc lamp housings. These are still supplied with a large number of new microscopes and can be used to illuminate Mosaic for AI. These broadband light sources deliver wavelengths from UV to IR and are adapted for use with Mosaic using an electro-mechanical shutter and excitation filter to select the AI wavelength range. They can also be used for their original purpose of Epi illumination in Mosaic and MicroPoint. When used with Mosaic the rich UV output can be used for localized release of caged compounds and other wavelengths can be used for activation and bleaching.
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