Active Illumination

FRAP, Ablation, Photoactivation, Uncaging and Thrombosis - Application Movies

The following gallery of movies demonstrates the depth and breadth of life science applications addressed with Andor's active illumination systems.

Active Illumination: Laser Induced Thrombosis
	This movie was provided by the Bruce Lab at the Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School. This movie shows in vivo laser induced thrombosis in mouse cremaster muscle. Images were acquired in real time on a two color confocal recording platelet deposition and fibrin generation.
Active Illumination: Photoactivation - Compartmentalized Dynamics
	This Mosaic movie shows highly soluble compartmentalized PA/ GFP simultaneously released in the cytoplasm and nucleus.
Active Illumination: High Speed FRAP Kinetics
	Images acquired using the MicroPoint to FRAP soluble GFP in a live cell.
Active Illumination: Diffraction Limited Photoactivation with TIRF
	This Mosaic movie was acquired while simultaneously photoactivating GFP in multiple ROI's while acquiring images in real time on a Nikon TIRF system. This movie demonstrates that the Mosaic can target and photoactivate multiple very small regions, the smallest at the diffraction limit.
Active Illumination: Ablation - Micro-surgical Cell Membrane Cut
	This MicroPoint movie shows a real time microsurgical ablation of cell membrane in a drosophila embryo. Power at the specimen plane was precisely controlled in order to cut the membrane without blowing it apart.
Active Illumination: Ablation - Cell Wounding
	Laser induced wounding in Drosophila embryo. This movie was captured in real time while using the MicroPoint Laser System for ablation.
Active Illumination: Photoactivation in C.Elgans
	This Mosaic movie was provided by Jim Priess, Howard Hughes Investigator at Fred Hutchinson Cancer Research. The movie shows GFP/ Photoactivation in a large ROI in P-granules in C-Elegans gonad while acquiring real time confocal images and monitoring movement.
Active Illumination: GFP Photoactivation
	This lamp based Mosaic movie was acquired during a Physiology Course at Woods Hole. The movie shows simultaneous Photoactivation of GFP in multiple ROI's in a fibroblast cell while acquiring images in real time and diffusion without a delta time.
Active Illumination: Uncaging - Calcium Release
	Calcium Release in Flou-4 using Mosaic with 405 laser on confocal microscope acquiring images of calcium sparks in real time.
Active Illumination: FRAP in C.Elgans
	This Mosaic movie was provided by Jim Priess, Howard Hughes Investigator at Fred Hutchinson Cancer Research. The movie shows simultaneous FRAP of several Nuclei in a C-Elegans embryo captured on a spinning disc in real time, without a gap time between acquisition and the bleaching in each individual cell.
Active Illumination: GAG-GFP Photoactivation for Particle Tracking
	This Mosaic movie was provided by Paul Spearman MD., at Emory. A punctuate population of PA/ Gag/GFP- the major structural protein of HIV- is activated in order to study its movement by particle tracking. The Mosaic was used to simultaneously target two areas, one around the nucleus and another at the cell membrane boundary, while acquiring images in real time on a spinning disc confocal running green and red channels.
Active Illumination: GAG-GFP Photoactivation for Particle Tracking
	This Mosaic movie came from Margaret Gardel's lab, University of Chicago, courtesy of Yvonne Aratyn. This Human bone osteosarcoma cell expresses GFP in actin stress fibers and diffuse cytoplasmic actin in the cell center. The cell has two recovery components, a slow component in the actin stress fibers and a much faster component in the cytoplasmic cell center. 5 regions were bleached, four in the actin stress fibers and a large region in the cytoplasmic cell center. Every ROI is sharp with vertical walls and without any recovery gradient. Because one recovery event occurs so much faster than the other it is only possible to capture and analyze the relationship between these two structures with an illumination system that can simultaneously illuminate multiple regions of interest in real time with zero delta time.