Live Cell Confocal Upgrades

Andor’s Revolution range includes our laser spinning disk live cell confocal microscopy systemsutilizing the Yokogawa CSU. We have created a range of upgrade solutions for Perkin Elmer UltraView®and Visitech system users to bring a new lease of life to legacy systems.

Revolution Confocal setup

The Perkin Elmer (PE) UltraView® laser spinning disk confocal imaging system was first introduced in the mid 1990s by LSR (later acquired by PE). UltraView® systems have been developed and updated over the last decade and include LCI, RS and most recently ERS models. Visitech International also supplied a significant number of CSU systems over this period. All these UltraView® and Visitech systems used the Yokogawa CSU, a patented dual Nipkow spinning disk unit to provide confocal scanning. Unfortunately compatibility between LCI, RS and ERS systems was not maintained and so for many users there is no clear upgrade or update path. The Yokogawa CSU remains a powerful and flexible confocal scanner, but some other system components are by now sub-optimal and many users would like to benefit from recent advances in detector technologies, solid state lasers, AOTFs and/or software.

Laser Warning Label

Andor Technology is a global supplier of the Yokogawa CSU, we have integrated CSU 10 and 22 units into our Revolution laser spinning disk confocal systems. As a result we are able to offer not onlycomplete imaging solutions, but also upgrade options for most UltraView® and Visitech systems. This is possible because the Andor Revolution product family is constructed in a highly modular format, which can be adapted to suit specific customer needs.

Why Upgrade?

Dr Raplh Albrecht with colleagues Angki & Rainer

Dr Ralph Albrecht and his research colleagues, Angki and Rainer acquired Andor iQ and iXon 887-BV which has boosted the performance and power of their Zeiss-based CARV system. There delight is obvious and "the results are outstanding" according to Ralph

  • Superior quality cooled CCD detectors
  • Pioneering EMCCD technology in the form of our award winning iXonEM+ camera available in three formats for ultra-fast ion imaging (DV860BV - 128x128 pixels), high sensitivity back illuminated (DV897BV - 512x512 pixels), and high resolution virtual phase (DV885VP –1002x1004 pixels)
  • ALC-400 series - solid state laser combiner with integral AOTF for real time wavelength selection and intensity control.
  • PCU 100 series – precision control unit for system control and synchronization
  • Andor iQ – advanced multi-dimensional imaging software
  • PZT-100 - piezo stage for real time specimen Z control
  • Optical enhancement of CSU units - enhanced illumination uniformity, plan apochromat projection optics, Semrock ion beam sputtered fluorescence filters
  • High performance single and dual camera adapters for CSU emission coupling
  • A FRAPPA programmable illumination system

UltraView Upgrades

In order to satisfy the needs of researchers wishing to upgrade their UltraView® systems we have created a number of upgrade packages aimed at the LCI, RS and ERS systems. As well as making use of the existing CSU scanner, we can utilize Sutter Instruments Lambda-10 filter wheel systems, NEOS AOTF controllers, existing lasers and PIFOC and Piezo-Jena objective piezo focus controllers. For simplicity we have identified three packages as follows, but please note that other custom configurations can be provided.

UVU-L10

Aimed at older LCI systems, the L10 makes use ofthe Sutter filter wheels for excitation and emission selection and PIFOC piezo controller and assumes that existing lasers will be reused.

UVU-R10

UltraView® RS systems utilize the Prairie Technology laser combiner with NEOS AOTF controller for excitation control, Sutter L10 for emission discrimination and PIFOC for focus control.

UVU-R20

UltraView® ERS systems utilize the NEOS AOTF controller for excitation control, Prior Optiscan 10 for emission discrimination and Piezo-Jena piezo focus control. The R20 package leaves the ERS controller intact, and so requires an external Piezo-Jena piezo controller, while all other components can be used directly.

Visitech Upgrades

UVT-T10

Aimed at Visitech QLC (CSU) systems, the UVT-T10 makes use of the Visitech AOTF laser controller, Sutter filter wheels for emission discrimination and PIFOC piezo controller. It assumes that existing lasers will be reused. Specials may be supported, so please ask.

Discover New Ways of Seeing