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Precision Dual Port Camera Adapter (PDPC)

Figure 1 - Precision Dual Port Camera Adapter

The Precision Dual Port Camera Adapter (PDPC) was developed in collaboration with Dr Sajit Mayor and colleagues, NCBS in Bangalore, India. This group had especially demanding camera alignment requirements for anisotropy imaging. In this imaging mode a polarizing beam splitter is used to separate the S and P EM polarizations of the fluorescence image. Alignment must be precise to allow sub-pixel precision in estimating local polarization effects.

To meet the demanding alignment and stability requirements, one camera was mounted on precision XYZ stage and the other on a goniometer for rotational adjustment as shown in the Figure 1. The beam splitter cube assembly was redesigned to deliver +/-3 degrees of rotation about the Z and Y axes. This ensures the cube orientation is optimized for contrast between the S and P polarizations, while Y axis rotation allows for some control of the image position on the second camera. Once the cube is optimized, then the mechanical adjustment of the cameras is optimized using the XYZ and rotational controls and an camera alignment Wizard in Andor iQ.

Beam Splitter Cube provides +/-3 degrees of rotation adjustment

PDPD C and DPC configurations

The PDPC and DPC both operate in various optical configurations. These include:

• with a C or C-S lens
• directly on a microscope C side-port
• on a microscope C side-port with filter wheel
• on a microscope C side-port with filter wheel and CSU