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Microscopy Systems

Revolution® DSD

White light confocal microscopy system -
Performance breakthrough in live cell imaging

Andor is delighted to introduce the Revolution® DSD, an innovative white light confocal spinning disk based on ‘aperture correlation’ 1.

Unlike other white light confocal instruments, the DSD 2 (differential spinning disk) achieves a high degree of optical sectioning by active background rejection. This technique ensures that the DSD delivers high contrast, low background images with objective magnifications from 10x to 100x, using air water or oil immersion lenses, making it suitable for a wide range of live cell and developmental applications.

Andor has designed the Revolution® DSD system with our best in class interline CCD camera, Clara, new AMH 200 W, DC-stabilized metal halide source and Andor iQ workstation with optional Piezo Z stages. These components are integrated seamlessly to perform 5D and 6D imaging and the system can be fitted to all makes and models of inverted and upright microscopes.

BPAE triple labelled cells for DNA - DSD versus wide field performance

Mouse kidney section as seen with 63x, NA1.3 oil objective in widefield (left) and Revolution® DSD confocal (right).
Maximum intensity projections imaged with Revolution® DSD at exposure times: Red 0.5 s, Green 0.3 s, Blue 1.5 s.

FeaturesBenefits
Real-time control and viewingSwitch wavelength and mode in real time between wide field and confocal with one mouse click.
Full spectrum, laser-freeExcitation 340-800 nm using Andor’s 200 W, DC-stabilized, high intensity, metal halide source, AMH-200.
Excellent confocalityActive background rejection ensures strong, clear optical sections even at low magnification.
High throughputEfficient disk design delivers 50% transmission in high sectioning mode (HS) and 25% in high contrast (HC).
Highly cost effectiveRevolution® DSD is designed for high levels of performance with easy alignment and low maintenance.
Ideal for live cells and embryosOperating from 10X to 100X, makes DSD suitable for live cell, tissue and whole embryo applications.
Immuno, GFPs, ratio labelsBroad spectrum source and filter selection allows use of UV, visible and NIR optical probes.
Integrates with any microscopeDSD connects to any side port and we offer customized supports for upright designs. Flexibility is a key goal.
Andor Clara – best in class cameraClara delivers the best interline CCD camera performance with lowest read noise and extended spectral range.
AMH – high performance light source200 W DC stabilized, delivers twice the output of 120W models with 2000 hour, alignment-free lamp cartridge.
Active background rejectionPatented optical design combines in-focus and out-of-focus light to reject background and deliver high contrast.

1 Juskaitis, R., Wilson, T., Neil, M. A., Kozubek, M., Efficient real-time confocal microscopy with white light sources. Nature 1996, 383, 804-6.
2 DSD is manufactured by Aurox Ltd.

© 2010 Andor Technology plc.