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Andor Microscopy Systems Division Overview

Andor Revolution provides a framework for our range of laser-illuminated microscopy solutions, including spinning disk confocal, TIRFM and photobleach and activation modalities. Our spinning disk, live cell confocal instrument, illustrated below, combines Andor’s award winning iXon EMCCD cameras, solid-state laser combiner and the renowned Yokogawa CSU-X1 confocal scanner “inside”. Andor has established a global distribution agreement with Yokogawa Electric Corporation and harnessed our optoelectronics engineering and manufacturing knowledge to create powerful live cell confocal solutions. Andor's direct market presence and unique solutions bring you unrivalled performance, product understanding and support.

Revolution encompasses a range of components, both hardware and software that fit seamlessly together creating a complete laser microscopy solutions. An open flexible component focus also allows us to provide key pieces of hardware stand-alone. We want to tailor solutions matched to your needs. At the core of Revolution systems is Andor iQ, a multi-dimensional imaging software which synchronizes iXon EMCCD cameras with the CSU-X1 confocal spinning disk and other key hardware components such as Piezo Z100 z-stage and our Laser Combiner with AOTF for rapid laser line selection.

The Revolution Range offers you

• Ultimate sensitivity from combination of iXon^EM+ EMCCD and CSU-X1 spinning disk or TIRF illumination.
• Fast frame rates (up to 1000 sec^-1) with perfect synchronization
• Active laser blanking provides:
  - minimal photo bleaching - particularly vital for non-ratiometric dyes.
  - reduced phototoxicity of living cells
• Study live specimens with reduced fluorophore concentrations or expression levels
• Minimal perturbation of physiological events
• Synchronization of electrophysiology and photolysis with the Precision Control unit
• Rapid optical sectioning with Piezo Z100 for 4D imaging - up to 100 sections sec^-1
• Detection limit down to single GFP molecules in parallel at supra-video frame rates
• Photo-bleaching and activation to study diffusion and transport of labelled molecules
• Powerful visualization, image analysis and tracking tools in iQ software
• Detect and track many individual vesicles in parallel
• Align and merge optically split images in iQ
• Powerful tools for FRET visualization and analysis