Multi-Wavelength Imaging

The vast majority of imaging applications involve the use of more than one fluorescent probe e.g. FRET, dual color imaging, co-localization studies etc. For multi-wavelength imaging Andor Technology has the most flexible and versatile solution available to the researcher.

ModelDescriptionBuy it Now...
High performance, two camera imaging adapter
Optosplit II
Dual Emission Image Splitter for single camera.
Optosplit III
Triple Emission Image Splitter

Multi-Wavelength Portfolio


The TuCam, a new generation two-camera adaptor, for both macro and microscopic applications can be configured for simultaneous imaging from two similar cameras or as a switch between camera models with different imaging capabilities. It is compatible with Andor’s complete range of market leading low light imaging cameras including iXon Ultra and Luca EMCCDs, Clara Interline CCD and the Neo and Zyla sCMOS cameras, as well as any detector from a third party supplier. It is an easy to integrate solution for any laboratory or multi-user imaging facility and extremely user friendly.

The TuCam is an “off-the-shelf” solution and does not need to be integrated into any software. Having the largest aperture on the market means that a wide range of sensors from very small to very large can be used effortlessly with the TuCam.

Optosplit II/III

Another option is the Optosplit II/III. These are simple devices enabling a single camera to record images simultaneously at two or three optically different wavelengths. It is an inexpensive method to perform multi wavelength applications with one camera sensor. The Optosplit II/III will work with a wide range of imaging cameras.

Both devices can be added seamlessly to your microscope and will benefit your lab or research facility by providing more options and flexibility for the researcher.

Multimedia Library
Application Movies (2)
Product Photos (14)
Publications Database
Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution
Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes
Automated line scan analysis to quantify biosensor activity at the cell edge
A molecular signature of tissues with pacemaker activity in the heart and upper urinary tract involves coexpressed hyperpolarization-activated cation and T-type Ca2+ …
Selective trapping of single mammalian breast cancer cells by insulator-based dielectrophoresis
Single-Molecule Spectroscopic Imaging Studies of Polarity Gradients Prepared by Infusion-Withdrawal Dip-Coating
Heterogeneous Intracellular Trafficking Dynamics of Brain-Derived Neurotrophic Factor Complexes in the Neuronal Soma Revealed by Single Quantum Dot …
Shadowless-illuminated variable-angle TIRF (siva-TIRF) microscopy for the observation of spatial-temporal dynamics in live cells
Gilded nanoparticles for plasmonically enhanced fluorescence in TiO2: Sm3+ sol-gel films
Effects of commonly used antiviral vaccines on human plasmacytoid dendritic cells
Molecular length dependence of single molecule wobbling within surfactant-and solvent-filled silica mesopores
Investigation of Fluorescence Emission from CdSe Nanorods in PMMA and P3HT/PMMA Films
Fast structural responses of gap junction membrane domains to AB5 toxins
Focus on Super-Resolution Imaging with Direct Stochastic Optical Reconstruction Microscopy (dSTORM)
Biarsenical ligands bind to endogenous G-protein α-subunits and enable allosteric sensing of nucleotide binding
Automated line scan analysis to quantify biosensor activity at the cell edge
Multicolour single molecule imaging in cells with near infra-red dyes
Formation of a Mast Cell Synapse: Fc «RI Membrane Dynamics upon Binding Mobile or Immobilized Ligands on SurfacesAmanda Carroll-Portillo
Live Imaging of Chemotaxis Protein Clusters in Escherichia coli
The impact of a contractile actomyosin system on reconstituted phase separated membranes