Optosplit II Image Splitter for Multiwavelength Imaging | Andor

Optosplit II

The Optosplit II image splitter is a simple and elegant device for dividing an image into two separate, spatially equivalent, components that can be displayed side by side on a single camera chip.

  • Cost effective - only one camera required
  • Dichroic mirror and emission filters mounted in readily interchangeable cube
  • Unity magnification fitted as standard
  • Simple intuitive alignment in x, y, and focal planes
  • Interchangeable mount for N.D. filter or chromatic correction lenses


One Camera Solution - Dual Emission Image Splitter

The Optosplit image splitter is sold by Andor as part of our dual wavelength imaging portfolio. It is a simple device enabling a single camera to record images simultaneously at two different optical wavelengths.

Traditionally an electronic filter changer or a second camera would be used, neither of which is ideal for some applications. The Optosplit has been designed as a convenient, inexpensive solution to simultaneous dual wavelength imaging. It has the unique feature of a rotating mirror cradle, which gives adjustable spatial separation, to facilitate image registration.

Device drivers are included in several commercial imaging packages to assist registration and to allow real-time and off-line ratioing or fluorescence overlays. Alternatively, the Optosplit can be used with simple image capture software and the processing carried out manually off-line. The simple and accessible design makes the Optosplit an excellent platform for alternative applications, such as dual polarization imaging. Whilst optimized for coupling to a scientific microscope, the Optosplit can also be used with camera lenses or any other system of lenses that produce an image plane of suitable size.

Features and Benefits

Features Benefits
Variable internal path separation Allows optimization of the internal optics to match the aperture of the optical system, therefore minimizing the introduction of aberrations.
Dichroic mirror and emission filters mounted in a readily interchangeable cube Allows the user to exchange filter sets both easily and quickly. Some competing products have factory fitted filters.
Variable and locking rectangular diaphragm aperture for defining field size Allows the user to define the ROI both horizontally and vertically and set the images to the optimum size for the camera sensor. Once set, the aperture can be locked in position
Compact design with integral C-mount input and output ports Advantageous where laboratory space is at a premium and the integral C-mounts allow it to be easily attached to a wide variety of standard microscopes and CCD cameras.
Simple and precise controls for image registration Allow the split images to be accurately and easily centred in the desired field of view, and pixels aligned with respect to each other.
Interchangeable filter / dichroic holders for dual and single wavelength imaging Flexibility to use multiple wavelengths by simply changing filter and re-sizing the defined field.
Aperture diaphragms to balance signal levels if appropriate Acts as an adjustable neutral density filter, which can be more convenient than using neutral density filters.
Rotating filter mount for polarization studies Accurately orientates the emission polarization to maximize the contrast between the two channels.



The Andor Learning Center hosts a wide range of case studies, technical articles and webinars to guide you through the many features of our portfolio.


A molecular signature of tissues with pacemaker activity in the heart and upper urinary tract involves coexpressed hyperpolarization-activated cation and T-type Ca2+ …
Selective trapping of single mammalian breast cancer cells by insulator-based dielectrophoresis
Single-Molecule Spectroscopic Imaging Studies of Polarity Gradients Prepared by Infusion-Withdrawal Dip-Coating
Heterogeneous Intracellular Trafficking Dynamics of Brain-Derived Neurotrophic Factor Complexes in the Neuronal Soma Revealed by Single Quantum Dot …
Shadowless-illuminated variable-angle TIRF (siva-TIRF) microscopy for the observation of spatial-temporal dynamics in live cells
Effects of commonly used antiviral vaccines on human plasmacytoid dendritic cells
Molecular length dependence of single molecule wobbling within surfactant-and solvent-filled silica mesopores
Investigation of Fluorescence Emission from CdSe Nanorods in PMMA and P3HT/PMMA Films
Fast structural responses of gap junction membrane domains to AB5 toxins
Formation of a Mast Cell Synapse: Fc «RI Membrane Dynamics upon Binding Mobile or Immobilized Ligands on SurfacesAmanda Carroll-Portillo
Live Imaging of Chemotaxis Protein Clusters in Escherichia coli
The impact of a contractile actomyosin system on reconstituted phase separated membranes

Drivers and Downloads


OptoSplit II
The Optosplit II image splitter is a simple and elegant device for dividing an image into two separate, spatially equivalent, components that can be displayed side by side on a single camera chip. Splitting is usually performed on the basis of wavelength, allowing applications such as ratiometric calcium imaging or FRET, however, polarizing beamsplitters are also supported. The two images can be captured simultaneously offering a major benefit over manual or electronic filter changers. The Optosplit II can significantly widen the scope of any fluorescence imaging system (0615 R1)