Zyla 4.2 PLUS sCMOS

> 80% QE and Industry Fastest Speed - SHIPPING NOW

What's new in Zyla 4.2 PLUS?Zyla 4.2 PLUS is the latest sCMOS technology advancement from Andor. At its heart, Zyla 4.2 PLUS benefits from latest generation sCMOS sensor technology, delivering a further 10 % boost in QE, resulting in an unprecedented 82% QEmax. The broad QE profile provides excellent coverage of the visible/NIR wavelength range, highly optimized to a wide range of common fluorophores. Further innovation has also introduced industry-fastest USB 3.0 frame rates and industry-leading response linearity of 99.8%, for unparalleled quantitative accuracy of measurement across the full dynamic range. Zyla 4.2 PLUS is ideal for applications such as Super-Resolution Microscopy, Ion Signalling Microscopy, LightSheet Microscopy, Line Scanning Confocal Microscopy and TIRF Microscopy.

Zyla 4.2 PLUS sCMOS Camera

The newest addition to the Andor sCMOS camera portfolio, Zyla 4.2 PLUS benefits from latest generation sCMOS sensor technology, delivering a further 10 % boost in QE, resulting in an unprecedented 82% QEmax. The broad QE profile provides excellent coverage of the visible/NIR wavelength range, highly optimized to a wide range of common fluorophores, the sensitivity boost permitting use of shorter exposure times, faster frame rates and reduced phototoxicity.

Superior data transfer efficiency and Zyla’s unique 12-bit high speed mode combine to deliver an incredible 53 fps through the super-convenient USB 3.0 interface, 77% faster than competing sCMOS cameras. With this capability, speed is on tap to allow users to follow faster dynamic processes with improved temporal resolution. Opt for the Camera Link version to access up to a blistering 100 fps (full resolution). Zyla 4.2 PLUS also offers industry-leading linearity (99.8%) for unparalleled quantitative accuracy of measurement across the full dynamic range.

The Zyla 4.2 is ideal for applications that benefit from optimal sensitivity and speed, such as calcium imaging, light sheet microscopy and super-resolution microscopy, as well as numerous high speed applications within astronomy. Zyla 4.2 PLUS can also output data at a sustained 26,041 fps from a 2048(h) x 8(v) ROI, ideal for measuring diffusion coefficients in Fluorescence Correlation Spectroscopy (FCS).

The ‘4T’ (4-transistor) design of the sensor pixel permits more photons to enter, driving Quantum Efficiency up to 82%. The 4T design also means that it is fundamentally a Rolling Shutter sensor. However, a feature called ‘Global Clear’ has been implemented, which allows for a simulated Global Exposure mode, which requires TTL communication between the camera and a pulsed light source, emulating the Global shutter exposure condition.

The Andor GPU Express library has been created to simplify and optimize data transfers from camera to a CUDA-enabled NVidia Graphical Processing Unit (GPU) card to facilitate accelerated GPU processing as part of the acquisition pipeline.

Key Specifications
Sensor Type Front Illuminated Scientific CMOS (4T) – sCMOS 2.0
Active Pixels 2048 x 2048 (4.2 Megapixel)
Sensor Size 13.3 x 13.3 mm (18.8 mm diagonal)
Pixel Size 6.5 μm
Pixel readout rate (MHz) Fast Read 540 (270 MHz x 2 sensor halves)
Slow Read 216 (106 MHz x 2 sensor halves)
Read Noise (e-)
216 MHz
560 MHz
 
0.9
1.10
Maximum frame rate 53 fps @ full frame (USB 3.0)
100 fps @ full frame (Camera Link 10-tap)
Maximum Quantum Efficiency 82%
Dark current, e-/pixel/sec @ min temp 0.14
Readout modes Rolling Shutter
Pixel well depth 30,000
Maximum dynamic range 33,000:1
Linearity 99.8%
Photon Response Non-Uniformity (PRNU) < 0.1%
Pre-defined Region of Interest 1920 x 1080, 1392 x 1040, 512 x 512, 128 x 128
Data range 12 bit (fastest frame rates) and 16 bit (max dynamic range)
Interface options USB 3.0, Camera Link 10-tap
Features Benefits
NEW > 80% QE Highest and broadest available sCMOS QE. Optimized to a wide range of common fluorophores. Enables shorter exposures, faster frame rates and reduced photobleaching.
0.9 e- read noise Lowest read noise sCMOS. Significantly lower than any CCD.
NEW Industry Fastest Frame Rates Follow faster processes. Industry-fastest USB 3.0 frame rates, 53 fps (full frame). 100 fps (full frame) sustained via Camera Link.
4.2 megapixel sensor format and 6.5 μm pixels Delivers extremely sharp resolution over a 18.8 mm diagonal field of view; ideal for cell microscopy and astronomy.
LightScan PLUS Maximize fluorescence signal and confocality concurrently in applications such as Scanned Light Sheet Microscopy and Line Scanning Confocal Microscopy.
Ultra Low Fan Vibration Designed with vibration sensitive experiments in mind, such as super-resolution microscopy.
GPU Express Simplify and optimize data transfers from camera to Graphical Processing Unit (GPU) card to facilitate accelerated GPU processing as part of the acquisition pipeline.
Dual-Gain Amplifiers Maximum well depth and lowest noise simultaneously, affording extended dynamic range of 33,000:1
12-bit and 16-bit modes 12-bit for absolute fastest frame rates through USB 3.0; 16-bit for full dynamic range.
Compact and Light Ideal for integration into space restrictive set-ups. Ideal for OEM.
Water Cooled Option Zyla 5.5 and Zyla 4.2 available as water cooled variant, for absolute lowest vibration and -10°C cooling / reduced darkcurrent.
TE cooling to 0° C in 27 °C ambient Ideal for OEM integration into enclosed systems.
Extensive FPGA on-head data processing Essential to ensure best image quality and quantitative fidelity from sCMOS technology.
Hardware Timestamp FPGA generated timestamp with 25ns accuracy.
Dynamic Baseline Clamp Essential to ensure quantitative accuracy across the image area and between successive images of a kinetic series.
Graphs and Drawings
QE Curve
QE VS. Flurophore Emissions
 Dimensions - Front
 
 Dimensions - Side
 
 Connector Panel - Std Camera Link and USB 3.0

Drawings for Zyla 4.2 water-cooled version also available
Advanced Features

Fast Frame Rates

The Zyla 4.2 sCMOS is capable of delivering 100 fps sustained, the data streaming to PC through a high-bandwidth ‘10-tap’ Camera Link interface. A market leading, cost effective USB 3.0 version is also available, delivering 53 fps (full frame), faster from ROI.

The sCMOS sensor in Zyla 4.2 has highly parallel readout architecture. All 2048 columns possess their own Amplifier and Analogue to Digital Converter (ADC), at both the top and bottom of the column. This means that not only are all columns read out in parallel, but the readout direction of each column is split in the center, the signal from top and bottom halves.

  Zyla 4.2 USB 3.0 Zyla 4.2 Camera Link
Array Size Rolling Shutter Rolling Shutter
2048 x 2048 53 (40) 100 (100)
1920 x 1080 107 (80) 192 (192)
512 x 512 403 (403) 403 (403)
128 x 128 1578 (1578) 1578 (1578)

Zyla 4.2 frame rates across different ROI sizes; 12-bit (16-bit) modes

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Lowest Noise Floor

Andor’s ultrasensitive Zyla 4.2 sCMOS camera offers an unparalleled 0.9 electron (median) typical read noise floor, achievable at 40 fps, representing 216 MHz pixel readout. Furthermore, advancing readout to full speed has minimal impact on read noise, the Zyla offering a typical noise value of 1.1 electron (median) at 100 fps. For the best CCD cameras to even approach 2 electrons noise, a readout speed of 1MHz or slower is required. This minimal detection limit renders the Zyla 4.2 sCMOS suitable for a wide variety of challenging low light, moderate/high speed imaging applications when compared to CCD cameras.

To learn more, download the Andor sCMOS Brochure to access the technical note entitled ‘Understanding Read Noise in sCMOS ’.

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Extended Dynamic Range

The innovative Dual Amplifier architecture of the sCMOS sensor in Zyla 4.2 uniquely circumvents the need to choose between high or low gain amplifiers, in that signal can be sampled simultaneously by both high gain (low noise) and low gain (high capacity) amplifiers. As such, the lowest noise of the sensor can be harnessed alongside the maximum well depth, affording widest possible dynamic range. Uniquely for such a relatively small pixel design, this allows for dynamic range performance of 33,000:1 in Zyla 4.2.

Rolling and Global Shutter
High contrast image of LED illuminated picture, captured with Neo camera. The zoomed region shows pixel regions that are sampled by both high gain (low noise) and low gain (high capacity) amplifiers respectively.

To learn more, download the Andor sCMOS Brochure to access the technical note entitled ‘Dual Amplifier Dynamic Range ’.

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Superb Linearity (99.8%)

New on-camera intelligence delivers a significant linearity improvement, providing unparalleled quantitative measurement accuracy across the full dynamic range.

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Superior Image Quality

A lot of intelligence has been directed towards delivering best image quality and uniformity in the Zyla. The FPGA stores offset compensation maps at the pixel level (as opposed to column level) for different combinations of gains and readout speeds, thus minimizing fixed pattern noise. Then, a further set of real time algorithms provide compensation for any further dynamic fluctuations. Gain compensation maps adjust for any minor differences in pixel responsivity, resulting in a Photon Response Non-Uniformity (PRNU) specification of < 0.5%.

These attributes, combined with markedly superior performance and similar pricing, places Zyla sCMOS as the ideal camera with which to upgrade from existing interline CCDs.

Superior Image Quality
Andor’s Dynamic Baseline Clamp affords superior background image quality (Dark image, Rolling Shutter, 10 ms exposure)
Column Structure
Column structure reduction algorithm, inherent to Zyla and Neo sCMOS cameras, minimizing fixed pattern column-level noise from the low gain (high capacity) channel

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Dynamic Baseline Clamp

The Dynamic Baseline Clamp was developed by Andor specifically for their sCMOS cameras. This real time algorithm uses available dark reference pixels on either side of each row of the sensor to compensate for any real time variation of the baseline (bias) offset.

  • A flat baseline offset across the entire image
  • Baseline is rigidly clamped between all images of a kinetic series
  • Quantitative reproducibility of data taken in different sessions
  • Significantly improves image quality

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Rolling Shutter & Simulated Global Shutter

The Zyla 4.2 differs fundamentally from Zyla 5.5 in terms of shutter flexibility. Whereas Zyla 5.5 offers both Rolling and True Global shutter modes, the Zyla 4.2 operates in Rolling shutter mode. However, a mechanism called ‘Simulated Global Exposure’ is available, whereby a TTL output from the camera can be used to activate a pulsed light source, emulating the Global shutter exposure condition, albeit with less efficiency.

  • Rolling shutter essentially means that different lines of the array are exposed at different times as the read out ‘wave’ sweeps through the sensor, a row in the middle starting the exposure at least 10ms before rows at the edges. The lowest readout noise and fastest frame rates are available from this mode (see the video) .
  • Rolling and Global Shutter
    Rolling Shutter exposure sequence (single frame)
  • Simulated Global shutter is an exposure mechanism that can be accessed using Zyla 4.2, as a means to emulating the Global Shutter and avoiding possibility of spatial distortion when imaging moving objects. Note however that True Global Shutter is a more efficient mechanism. To access Simulated Global Exposure, the ‘FIRE ALL’ TTL output of the cameras is used to activate a pulsed light source to illuminate only during the phase of the Rolling shutter exposure cycle during which nothing is ‘transient’, i.e. the middle section of the above Rolling shutter schematic.
  • Achieving 50 fps with Simulated Global Shutter, using the Fire ALL TTL output of the Zyla 4.2 to activate a pulsed light source during the non-transient phase of the Rolling shutter exposure cycle, resulting in 50% dead time. Note, The True Global Shutter mode of the Zyla 5.5 can achieve 50 fps with 100% photon collection and zero dead time, and does not require a pulsed light source.

To learn more, download the Andor sCMOS Brochure to access the technical note entitled ‘Rolling and Global Shutter ’.

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GPU Express

The Andor GPU Express library has been created to simplify and optimize data transfers from camera to a CUDA-enabled NVidia Graphical Processing Unit (GPU) card to facilitate accelerated GPU processing as part of the acquisition pipeline. GPU Express integrates easily with SDK3 for Andor sCMOS cameras, providing a user-friendly but powerful solution for management of high bandwidth data flow challenges; ideal for data intensive applications such as Light Sheet Microscopy, Super-Resolution Microscopy and Adaptive Optics.

  • Enhanced convenience, afforded by simple, optimized GPU data management
  • Guaranteed optimal data throughout
  • Superb, easily accessible documentation and examples.

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Spurious Noise Filter

Andor’s Zyla 4.2 sCMOS camera comes equipped with an in-built FPGA filter that operates in real time to reduce the frequency of occurrence of high noise pixels. This real time filter corrects for pixels that are above 5 electrons RMS and would otherwise appear as spurious ‘salt and pepper’ noise spikes in the image.5.5

The appearance of such noisy pixels is analogous to the situation of Clock Induced Charge (CIC) noise spikes in EMCCD cameras, in that it is due to the fact that we have significantly reduced the noise in the bulk of the sensor that the remaining small percentage of spuriously high noise pixels can become an aesthetic issue. The filter employed dynamically identifies such high noise pixels and replaces them with the mean value of the neighbouring pixels.

Demonstration of effect of Spurious Noise Filter on a dark image, 20 ms exposure time, 560MHz readout speed.

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Hardware Timestamp

Andor’s Zyla 4.2 sCMOS offers an FPGA generated hardware timestamp, coincident to the end of exposure with 25 nanosecond accuracy, essential to maintain accurate kinetic information relating to image capture.

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Multimedia Library
Application Images (2)
Application Movies (1)