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Imaris from Bitplane

Imaris 7.1 brings significant enhancements to pivotal key features within the software. New capabilities include filament tracing with 4D spine and dendrite tracking, a track editor and statistical annotations for 4D objects. Building on the success of Imaris software to date, these new features will enhance the performance of existing Imaris systems.



Real-time Volume Rendering for Life Sciences

Cutting-Edge 3D and 4D Imaging Software for Life Sciences

Andor is proud to announce the recent acquisition of Bitplane, the leaders in volume rendering, visualization and data analysis software. The Imaris product line offers the most powerful and versatile 3D and 4D imaging analysis software solution on the market for researchers in life sciences, medicine and related fields. Eight unique modules can be individually combined to perfectly match the researcher's imaging needs. From visualization and analysis to publication, Imaris provides the most user friendly, accurate and integrated solution when only excellence will do.

The Imaris core software module delivers all the necessary functionality for visualization, segmentation and interpretation of 3D and 4D microscopy datasets. Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size, from a few megabytes to multiple gigabytes in size. Conveniently load, process and visualize images acquired from almost any confocal and wide field microscope, including from Andor's Revolution live cell microscopy systems live cell microscopy systems, to gain new and groundbreaking insight from your image data.

Features and Benefits

Features Benefits
MIP (Maximum Intensity Projection) This projection is implemented in Imaris so that even the largest datasets can be interactively rotated in 3D or 4D space.
Blend Projection Weights the data that is closer to the viewer, so that on a 2D screen the viewer can get a more realistic 3D impression of the data set and the depth relationship of the objects being viewed.
Real-Time Shadow Rendering The fast hardware driven shadow projection can produce shadows on the three planes of the “Frame Object” to enhance the realism of the images and introduce additional depth perception.
Normal Shading Similar in appearance to surface rendering, “Normal Shading” does not require the computation of a triangle mesh. Instead it computes the orientation of the virtual surface from the voxels directly and uses this information to shade the surface appropriately. This allows you to carry out fast and flexible surface rendering for very large datasets
Display Adjustment The intensity for each color channel in the image can be interactively adjusted in real-time to eliminate background noise and to brighten the image. The gamma for each channel can also be interactively adjusted. For rendering techniques that utilize transparency, this characteristic can also be changed in real time.
Automatic Iso-Surfaces “Surface Objects” are created on an intensity value on a per channel basis. Selection of the threshold is completely interactive.
Automatic Local Contrast Surfaces “Surface Objects” are created based on the contrast in the area around the object. This allows the accurate section of objects where the overall intensity many vary within the depth of the image or over time. Selection is completely interactive.
Automatic Region Growing “Surface Objects” are created by first identifying a region to look in for the objects of interest using either the local contrast or iso intensity methods, then “Seed Points” are calculated to mark each individual object. The result is that complex images, where the out of focus light overlaps between what should be separate objects, can have the objects split apart and treated individually.
Retain various sets of surface creation parameters save and remember surface object creation process parameters to use identical settings for analysis of another data set
Surface Scissors Manually split two touching “Surface Objects” along an interactively specified cutting plane. One click separates the objects.
Semi-Automatic Surface Generation The “Magic Wand” allows automatic global surface creation based on a manually selected ROI. The “Marching Cubes” algorithm allows automatic surface additions only in selected local regions.
Surface Visualization “Surface Objects” can be visualized with the original data for verification purposes. The surface can be colored any desired color and given visualization properties for shininess and transparency. Visualization color properties can be selected from a predefined color palette or can be custom designed and saved for future use.
Color Coded Values Choose a statistical parameter and color code your statistical values based on this statistic for visual clarity.
Spots Like the “Surface Object” the “Spots Object” is an artificial object allowing the comparison between the original data and what has been segmented. Like the surface object, the spots object acts as a container from which statistics can be calculated with the Imaris MeasurementPro module and tracking can be performed with the ImarisTrack module. The difference between a “Spot Object” and a “Surface Object” is that a “Spot Object” is always spherical, but can vary in diameter.
Automatic Creation of Spots Spherical models of objects can be created automatically based on the approximate size of the object regardless of the overall intensity. This is ideal for images that have attenuation of intensity in Z or over time where pure intensity based methods fail.
Manual Creation of Spots Spots may be manually added or deleted in any image in 3D or 4D by simply moving the mouse over an object and clicking or double clicking. This allows for quick and easy editing if automatic detection has problems or for the possibility to do the work completely manually. Imaris assists the user with spot placement by automatically detecting the correct depth to place the spot into the screen saving the user tremendous amounts of time. When combined with “InMotion”, object identification and segmentation that previously took hours is completed in minutes.
Retain various sets of spots creation parameters save and remember spot object creation process parameters to use identical settings for analysis of another data set
Visualization of Spots Spots can be visualized together with any of the other available objects and with the original data.
Advanced On-Line Interaction With The Object Rotating, zooming in and out of the object and flying through the volume in real-time.
Kinetic Depth Effect Imaris offers a series of motion animation and depth cueing tools that are unique in the microscopy market and enhance 3D interaction. “InMotion” introduces stationary or animated visual elements into the visual data scene to provide a structured frame of reference if needed. The “InMotion” function allows the user to accurately visualize and easily select data in even the most complex 3D or 4D images.
Navigation Window A Navigation Window displays an overview of the data set in the “Surpass View”. While exploring datasets, you will never be lost because as you move within the data set the overview image updates in real-time.
Filtering The “Object Filtering” is incorporated into the creation process of surfaces and spots enabling the composition of filters consisting of several criteria and interactive visual feedback on parameter selection.
Block-Wise Paging The handling of huge data sets is based on the principle that only the parts of the dataset and the resolution of the dataset needed for the actual view are loaded to RAM and displayed. This principle makes the most efficient use of RAM memory space.
Processing Load, open and render huge images instantaneously and easily. If the computer system has multiple hard disk drives, Imaris can use all of them in parallel to transfer data that cannot be held in RAM or graphics memory. This results in improved performance for the largest datasets.
Key Frame Animation The “Key Frame Animator” allows creation of a movie from key views (key frames) in the image. It interpolates frames between the user-defined views to create a smooth movie with only a few keystrokes. Simply move the image to the desired position and press the add button to build a movie quickly.
Generate Storyboard The “Key Frame Animator” stores object visibility states, such as brightness and contrast, color, “Clipping Plane” position and more, in addition to the camera position and the time point. This feature allows the generation of small storyboards and creation of complex movies.
Pre-Defined Rotations For those that want a simple movie, just click one of the pre-defined movie buttons to make a simple rotation about a given axis.
Share Your Results create movie files on any platform and easily export these 3D and 4D movies as a QuickTime, AVI, MPEG or Flash files with the press of a button. The frame rate and compression settings can be easily controlled.
Select movie size choose your movie format from a comprehensive list - from screen image size to 1080p HD format and more.
QuickTime-VR Movie Record a QuickTime-VR movie of anything that is displayed in the 3D view of Imaris. A QuickTime-VR allows the 3D interactive rotation of the resulting image by anyone who has QuickTime outside of Imaris, allowing easy collaboration with other researchers.
Z-Movie Record a simple movie of playing up and down through the different focal planes of your Z stack.
Multithreading Imaris is able to utilize multiple cores and / or multiple processors to significantly improve the speed and performance of the software. Most image processing functions and object creation (segmentation) steps are capable of utilizing multiple threads. You can set the number of cores or processors that Imaris can use.
High Resolution Rendering Imaris allows creation of high-resolution images, larger than the resolution of the computer screen for use in publications and poster production.
Hardware Driven Computation Shadow Rendering and most other visualization processes are computed by the graphics processing unit (GPU) to improve the performance of Imaris.
Selective Clipping Planes The “Oblique Slicer” selectively cuts away objects on one side of the plane. It can be freely moved and rotated in the scene and allows you to look inside any object at any angle and any depth.
Multiple Light Sources A “light source” serves to illuminate objects in the viewing area and gives a more 3D appearance through shadows that are cast and reflections on the object for an image presented on a 2D screen. Applying multiple light sources to your image creates spectacular 3D objects because it highlights fine surface details that may otherwise be hidden.
3D Holographic Rendering Modes A “light source” serves to illuminate objects in the viewing area and gives a more 3D appearance through shadows that are cast and reflections on the object for an image presented on a 2D screen. Applying multiple light sources to your image creates spectacular 3D objects because it highlights fine surface details that may otherwise be hidden.
Basic Editing Imaris allows the addition or deletion of channels, slices, and/or time points.
Re-Sampling In Space And Time Data sets can easily be re-sampled. Re-sampling in 3D reduces the voxel density in a data set, re-sampling in 4D total reduces the number of time points. Resampling can happen before the data set is opened or once the dataset is already open in the program and therefore speeds up image data processing.
Free Rotation tool If your 3/4D image stack was acquired from a less then optimal point of view, for example due to unfortunate embedding and positioning of the sample, it can now be arbitrarily rotated in three dimensions. By resampling the image along your preferred axis of view it is now possible to access all the hidden information of your specimen’s rendering. This may help you freely reorient your sample in 3D so that all your samples have identical orientation when it comes to comparing various samples between different experiments.
Crop Images In Space And Time Cropping the data set in 2, 3 or 4D allows re-sizing of the images down to a region of interest (ROI), making it easier and faster to handle the viewing and storing of the images. The total number of time points can be reduced at the beginning or end of the series of images. Cropping of the dataset has a real-time preview and can be completed both before opening a dataset or once a dataset has been opened.
Subregion Processing By setting a customized subregion Imaris can remain interactive even when working on very large images. Interactively define the subregion in a 3D view and create Spots and Surfaces only within one or more sub ROIs. Once all the correct processing parameters have been determined for the sub region(s), the algorithms can be automatically run on the entire dataset.
Volume Painter Drawing disks, spheres, or hollow spheres into the selected image channel, Imaris uses the “Volume Painter” to manually clear selected areas in the volume image. Additionally, the “Volume Painter” allows adjustment of the voxel intensities before segmentation is completed with Surfaces, Spots, or Filament Objects.
Image Processing Tools Imaris contains several basic image-processing tools to aid in basic visualization and image analysis. These tools include 3D Gaussian and Median filters, Baseline and Background Subtraction, and Threshold Cut-off, all which can be used to refine the dataset.
Loading Time based images (2D+Time or 3D+time) can be loaded from all native file formats as well as TIFF and BMP images. If time stamp calibration is available with the original image it is utilized. Users have the option to time code non-calibrated images within Imaris.
Time-Slider The Time Slider is visible in all display modes (Slice, Section, Gallery Easy 3D, and Surpass). It allows viewing of a particular time point or to automatically play images through time simply by moving the time slider or pressing the play button.
Identical Interface All visualization, image processing, display adjustment, segmentation, and object creation steps can be carried out in the exact same manner on 4D images as they are on 3D images.
Scale Bar Imaris includes an automatically adjusting scale bar that is present in the “Slice”, “Section”, “Gallery”, and “Easy 3D” views. This scale bar can be resized and moved interactively based on each users needs.
Grid In the Surpass view a grid behind a 3D image can be turned on to provide information about the size of its structures. The properties of the grid can be edited to change color, grid spacing, font, shading, etc.
Image Axis The image axis can also be displayed to simplify orientation of the data set.

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Specification Sheets

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