Andor iQ3

Andor iQ is our flagship live cell imaging software. iQ - image and quantify - occupies a central role in our Revolution confocal and Photostimulation product range. iQ provides optimized control of Andor's award winning iXon EMCCD and sCMOS cameras in synchronized combination with a number of additional imaging hardware devices, addressing a range of bioimaging applications. Use iQ to run experiments to investigate dynamic processes in single cells through to multi-cellular systems, perform routine imaging of fixed samples, and generate 3 dimensional image volumes for analysis.

  • New Simplified 6D user interface for rapid setup and tuning
  • New User management – per user settings and ImageDisk
  • Dedicated Photostimulation device control – Ablate, Bleach, Activate
  • “Flexible Protocols” for advanced routines
  • Improved integration with Imaris

Andor iQ Software

Windows 7 Compatibility

Andor iQ is designed from years of experience and understanding of the demands that capturing highly dynamic cellular processes impose. iQ integrates a range of additional hardware for multi-parameter and multi-modal imaging, including devices such as confocal scanners, light sources including our own laser combiner, filter wheels and x,y, & z motorized stage control. Automation of this hardware with image capture is tightly controlled for optimum capture rates, and to minimise unnecessary light exposure for prolonged live cell imaging.

Recognizing that most biologists do not have time to learn a complex user interface, and that live biology frequently demands immediate attention, iQ3 provides a radical new user interface designed with workflow and rapid configuration in mind. In addition, IQ3’s new user management capabilities make life easier for both the user and the facility manager to create and manage personal settings, protect experimental configurations and images between users, and track time spent on systems for auditing. For all users of iQ this means a more efficient work flow and better productivity.

In addition to providing an Andor system solution, iQ can also be independently used with our Photostimulation devices dedicated to light manipulation techniques such as optogenetics, optophysiology, photobleaching (FRAP), photoactivation, photoconversion, ablation, uncaging and DNA damage. iQ will control these devices when fitted to 3rd party systems that have no dedicated software support of their own, capturing images from the host software for calibration and targeting.

iQ can be used for a range of bioimaging applications such as 3D live cell imaging, TIRF, ion imaging, bioluminescence and FRET. This makes iQ ideal for many research fields including cancer research, developmental biology, neuroscience, embryology, stem cell, and plant biology; capturing images from small subcellular components such as vesicles up to whole samples such as zebrafish and drosophila embryos. Continuous development and improvement ensures that Andor iQ represents a powerful and flexible core for live-cell imaging systems.

Features Benefits
6D user interface Single GUI to capture multi-dimension image series. Time efficient workflow for rapid parameter setting and minimal user training.
User management Store settings such protocols, channels, data storage locations per user. Protects user settings to ensure nothing is over written. Experiments and illumination settings can be shared between users via export/import.
Account tracking Records activity based on users and hardware configurations. Track the time users spend on a system for auditing purposes (e.g. for charging hourly rate).
Flexible Protocols Advanced interface available, on selection, for complex multi-modal imaging routines. Provides a programmable tree structure to build complex imaging experiments for those with more demanding application requirements.
Dedicated ImageDisk™ Proprietary storage solution. Saves data direct to hard disk avoiding Windows file swapping. Handles large data sets with ease, providing fast data review and manipulation
On-line Charting Quantify data as it is captured. Histogram and line profiling for single or multi-channel data on live or stored data.
3D/4D Viewer 3D, multi-channel, maximum intensity projection view. Provides quick review of data in 3D volume, including through time series.
FRAP tools Image capture and analysis features for photobleaching experiments. Dedicated, published, algorithms for the analysis of cell dynamics using the FRAP technique.
Other analysis tools Ratiometric algorithm-based analysis for ion imaging. Kymograph tool, multi-channel alignment, Image Math. A range of tools are provided for core imaging techniques used to evaluate cell and system biology. Providing fast feedback on experiments.
Python compatible Supports use of Python scripts Interface with iQ from other software tools and tailor functionality according to your needs.
Movie maker Tools for creating movies from captured experiments. Complex movies can be made merging different dimensions (e.g. time and then volume) for data review and presentation.
Virtual Camera Runs from image captured from desktop window or webcam-like source Permits the use of iQ to drive complimentary hardware components (e.g. Photostimulation device) along-side non-Andor imaging systems

Additional features include new multi-field scan dialogue and channel selection and sorting.

Multi-Dimensional at its Core
Andor iQ is multidimensional at its Core. This means you can handle multi-channel, time-series, Z-series and multi-field data with consummate ease. iQ provides easy to use tools for analysis, processing, visualization and management of live cell experiment data and "understands" dimensionality, so navigating and manipulating complex data sets is instant and interactive.
Handle Huge Data Sets With Ease
Andor iQ is built to handle huge data sets seamlessly with ImageDisk™ technology. ImageDisk™ has been developed and proven over more than ten years and is at the core of iQ's multidimensional memory management. ImageDisk™ extends your system memory to include fast hard drives and even RAID arrays so that you are never limited by RAM.

A smart, pre-emptive disk swapping algorithm monitors memory levels and current frame requirements during all acquisition and processing operations. It avoids Windows™ file swapping and handles frame access seamlessly, making sure the data you need is always available. Gigabit data sets are no longer a problem to acquire or manipulate and visualize.

Modules
Multimedia Library
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Application Movies (29)
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Publications Database
Gap geometry dictates epithelial closure efficiency
Direct interaction between centralspindlin and PRC1 reinforces mechanical resilience of the central spindle
The role of Ca2+ influx in spontaneous Ca2+ wave propagation in interstitial cells of Cajal from the rabbit urethra
Regulation of Patterned Dynamics of Local Exocytosis in Growth Cones by Netrin-1
A mouse model of human primitive neuroectodermal tumors resulting from microenvironmentally-driven malignant transformation of orthotopically transplanted radial glial cells.
Neuronal activity and AMPA-type glutamate receptor activation regulates the morphological development of oligodendrocyte precursor cells
Systematic imaging reveals features and changing localization of mRNAs in Drosophila development
Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity
Signal inhibition by a dynamically-regulated pool of mono-phosphorylated MAPK
A High Throughput Micro-Chamber Array Device for Single Cell Clonal Cultivation and Tumor Heterogeneity Analysis
A geminivirus betasatellite damages the structural and functional integrity of chloroplasts leading to symptom formation and inhibition of photosynthesis
Neuronal serotonin release triggers the heat shock response in C. elegans in the absence of temperature increase
RGS Proteins and Septins Cooperate to Promote Chemotropism by Regulating Polar Cap Mobility
A mechanical microcompressor for high resolution imaging of motile specimens
Motif‐Designed Peptide Nanofibers Decorated with Graphene Quantum Dots for Simultaneous Targeting and Imaging of Tumor Cells
In situ biosynthesis of fluorescent platinum nanoclusters: Towards self-bio-imaging guided cancer theranostics
Improvement of acquisition and analysis methods in multi-electrode array experiments with iPS cell-derived cardiomyocytes
Facile synthesis of fluorescent Au/Ce nanoclusters for high-sensitive bioimaging
… ) is a multifunctional protein that regulates quercetin, and impacts specific light and UV responses in the seed-to-seedling transition of Arabidopsis thaliana
Degradable ketal-based block copolymer nanoparticles for anti-cancer drug delivery: a systematic evaluation