Komet 7 and 7‑GLP

Komet software (now on Komet 7.0) allows the capture and analysis of images from ‘The Comet Assay’. The Comet Assay permits the quantification of DNA damage and repair in single cell preparations and is applicable to any eukaryotic cell. The assay can be used in both in-vitro and in-vivo testing and has been shown to be a powerful and sensitive predictor of genetic toxicity.

  • One-click capture and analysis of comets
  • Rapid scoring protocols
  • Computes all internationally accepted comet measurements
  • Pop-up controls accelerate scoring and minimize fatigue
  • Integrated creation of summary statistics

Komet 7 and 7-GLP - Acquisition and Analysis Software for The Comet Assay

The Comet Assay is a powerful tool for applications as diverse as cancer research, safety testing of pharmaceuticals and chemicals, environmental and occupational studies, dietary and even fertility research. Because the assay can be adapted to virtually any cell-type from almost any organism and study designs can be created to characterize DNA damage and repair as well as DNA integrity and comparisons between different populations, the assay is widely used in many different disciplines.

Since its inception in the 1980’s, the Comet Assay rapidly evolved as a valuable tool for research and safety testing. The adoption of the OECD Guidelines entitled “In-Vivo Mammalian Alkaline Comet Assay” (TG489) on September 26th 2014, marks a significant watershed as the assay is now officially embraced for safety testing among regulators responsible for human health and well-being.

In recognition of this important development, Andor Technology is proud to announce Komet 7 and Komet 7-GLP our latest versions of the original Komet software first released in 1992 by Kinetic Imaging. Komet 7 and Komet 7-GLP embrace new OECD requirements building on the experience developed over the last 20 years. Komet 7 maintains backward compatibility for hundreds of existing users, while also enabling new possibilities using the latest computer systems, cameras and light source developments. Komet is the most highly referenced comet assay analysis solution in research publications: invest in Komet for your DNA integrity research.

Features and Benefits
✔ Fast and easy to use – pop-up controls accelerate scoring and minimize fatigue.
✔ Proven performance – reliable data analysis with backward compatibility.
✔ User-friendly, freely distributed Database Viewer application.
✔ Databases include all comet images, parameters and audit trails.
✔ Integrated creation of summary statistics – optimizes data workflow and reporting
✔ Facilitates OECD TG 489 guideline requirements for data and statistical analysis.
✔ Fulfills FDA 21CFR part 11 requirements for electronic data.
✔ Certified Windows 7 compatibility - Windows 8.1 coming soon.
✔ Large Field of View options – score several cells per image.
✔ “Virtual Camera” – scores live images from ANY camera you already own.
✔ LED light sources - replace mercury bulbs with safe, efficient, long-life illumination.
✔ Complete solutions with the latest microscopes, cameras, workstations.
✔ Developed under ISO9001 quality assured engineering processes.
✔ Suitable for cancer research, toxicity studies and dietary research amongst others.
Package Description
KOMET-R-WSTN-Zyla Zyla 5.5 USB3, 5.5 MP, 6.5 μm pixel sCMOS camera with USB 3 connectivity, power supply and cables included. KOMET R and DBV pre-installed and burn-in tested. High Performance Dell T1700 8GB RAM, 512 GB SSD, Firepro performance Graphics, 24" LED Monitor. Full functionality guaranteed out of the box.
KOMET-GLP-WSTN-Zyla Zyla 5.5 USB3, 5.5 MP, 6.5 μm pixel sCMOS camera, with USB 3 connectivity, power supply and cables included. KOMET 7GLP and DBV pre-installed and burn-in tested. High Performance Dell T1700 8GB RAM, 512 GB SSD, Firepro performance Graphics, 24" LED Monitor. Full functionality guraranteed out of the box.
KOMET Komet R Software for imaging and analysis of comet specimens. Supplied with Andor Database Viewer for Data audit, archive and summary for reporting and statistical significance testing. Supports software licence. Andor camera support included - 3rd party see driver CD for purchase.
KOMET GLP Komet 7-GLP Software for imaging and analysis of comet specimens. Supplied with Andor Database Viewer for Data audit, archive and summary for reporting and statistical significance testing. Supports software licence. Andor camera support included - 3rd party see driver CD for purchase.
KOMET-WSTN Dell PC, T1700 with 8 GB RAM and 512 GB SSD installed with Windows 7 (64 bit) for use with Komet software. Included 24" Ultrsharp LED monitor (1920 x 1200).
KOMET-R-UPG Upgrade to Komet 7 with remote installation support. Requires the VID/USB adapter- see Komet Accessories.
KOMET-GLP-UPG Upgrade to Komet 7-GLP with new OECD compliance with remote installation support. Requires the VID/USB adapter- see Komet Accessories.

Accessories

Light Sources

LEDs (light emitting diodes) are replacing mercury lamps. Consider a safe, efficient and long lasting alternative, which can be fitted to any microscope. With a lifetime of 25,000 hours a LED source will last for more than 10 years of heavy daily use and pay for itself over a few years. Equally important, LEDs are not prone to flicker and instability, delivering better quality data. pE-300 white LED illumination unit. Provides a broad spectrum of illumination, UV peak at 365nm, covering the excitation bands of common fluorescent stains. Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%. Remote control is available via USB or TTL trigger.

Product Code Description
LL-PE300-WHT-NK1-I Suitable for Nikon Ti, TE2000, Eclipse 50-90i, E400-600, FN1, AZ100
LL-PE300-WHT-OL1-I Suitable for all Olympus scopes apart from BH2, LV200, LEXT, IMT
LL-PE300-WHT-LE1-I Suitable for all Leica compound microscopes (not stereo microscopes)
LL-PE300-WHT-ZS1-I Suitable for all Zeiss scopes

Converters required for video cameras to be used with Komet 7

If an analog video CCD is being used (e.g. Pulinix, Cohu, JVC) the following accessory will be required to allow images to be acquired with Komet 7 software

Product Code Description
KOMET-VID-USB2 Video-USB connector - The Imaging Source DFG/USB2pro converter, including USB2 cable and BNC adapter
IQ-VIRT-CAM Virtual Camera Driver required for Virtual Camera and Third Party Camera (not required for Andor camera)
Camera Support
Andor Zyla 5.5 USB3 Large field of view means faster scoring 
Andor cameras supported by older version of Komet Komet 7 is back-compatible with all Andor cameras previously supported by Komet 
Andor Virtual Camera Screen capture tool allows you to acquire from any camera you can run in Windows 7
Q-imaging camera (specify model and serial number) Confirm support of camera before purchase 
Analog video CCD (e.g. Pulinix, Cohu, JVC) Requires Windows-support Video-to-USB converter (see Accessories tab)
Publications Database
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Chloroquine-induced glioma cells death is associated with mitochondrial membrane potential loss, but not oxidative stress
CDC42 GTPase Activation Affects HeLa Cell DNA Repair and Proliferation Following UV Radiation‐Induced Genotoxic Stress
TGFβ1 Protects cells from γ-IR by enhancing the activity of the NHEJ repair pathway
Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays
Activation of DNA damage repair pathways in response to nitrogen mustard-induced DNA damage and toxicity in skin keratinocytes
Influence of oxidative stress, diaphragm fatigue, and
Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects
Catalytic antioxidant AEOL 10150 treatment ameliorates sulfur mustard analog 2-chloroethyl ethyl sulfide-associated cutaneous toxic effects
Effects of seven chemicals on DNA damage in the rat urinary bladder: A comet assay study
Mitogen-activated protein kinase signal transduction and DNA repair network are involved in aluminum-induced DNA damage and adaptive response in root …
Sodium arsenite induced changes in survival, growth, metamorphosis and genotoxicity in the Indian cricket frog (< i> Rana limnocharis</i>)
Hypoxia Provokes Base Excision Repair Changes and a Repair-Deficient, Mutator Phenotype in Colorectal Cancer Cells
Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay
Effects of Biovar I and Biovar II of< i> Ureaplasma urealyticum</i> on Sperm Parameters, Lipid Peroxidation, and Deoxyribonucleic Acid Damage in Male Infertility
MALDI-TOF fingerprinting of seminal plasma lipids in the study of human male infertility
Genotoxicity analysis of cerium oxide micro and nanoparticles in Wistar rats after 28 days of repeated oral administration
Sodium Fluoride Promotes Apoptosis by Generation of Reactive Oxygen Species in Human Lymphocytes
Genotoxicity assessment of cerium oxide nanoparticles in female Wistar rats after acute oral exposure
The differential localization of a methyl group confers to two triterpenes present in the olives a different anti-breast cancer activity.