Komet Software for Comet Assay - Data Outputs

Images, Intensity Profiles and Data Grid

• Print images and intensity profiles direct from Komet.
• Copy images to Windows Clipboard for use in other programs or reports.
• Save images to TIFF, BMP and JPEG formats.
• Apply Histogram Mapping and color palettes for intensity color mapping.

Figure 6 - Examples of comet images showing similar levels of damage, but using different visualization. The top image was acquired with a digital color CCD camera from a specimen labelled with SYBR-Green, showing how the cell would look in the microscope eyepiece. The bottom image has been pseudo- colored with a color scale in which blues represent low intensities ranging to green and red for higher values.

Komet Databases & Database Viewer

• When the user Saves to a Komet Database, the data and image of every comet scored is stored into a Database.
• Data output includes details of scoring session – user, time, date, protocol name and all settings.
• Database Viewer provides Decoding for blind-scored studies, essential for GLP purposes.
• Create and print a Gallery of comets for each specimen or treatment.
• Data can be organized by treatment group and statistical summaries produced for significance, exposure
• evaluation and dose response analysis.
• Optional locked Database provides security and validation of data.
• Create single specimen Histograms, multi-specimen Histograms (3D) and Response curves with error bars.
• Database Viewer can be distributed free of charge with the data for review at other sites.
• Integration and comparison of data from multiple databases.

Figure 7. Komet Database Viewer loads the scored datasets and displays the analysis data and image of each comet scored. Database Viewer lets you create a Gallery of comet images from a Database, as shown above. This Gallery can be easily printed or used for review and training.

Database Viewer - Share, Review, Train

• Share with colleagues - free distribution of software.
• Review results and explore data relationships.
• Train - show new users scoring and analysis procedures.

Figure 8a - Komet Database Viewer loads the scored datasets and displays the analysis data and image of each comet scored. This is the basic View/Review tool and you can scroll down the dataset and view the cells in a kind of “movie” by holding down the keyboard scroll down key.

Figure 9a (left) - 3D Histogram presents study data where a Dose Response was detected. The progression from Negative control (in grey) through the dose groups to the Positive control (orange) is apparent in the data distributions for Tail DNA. Figure 9b (right) - The data can also be presented in Response Curve format & Summary data table.